AMS1/YGL156W Summary Help

Standard Name AMS1 1
Systematic Name YGL156W
Feature Type ORF, Verified
Description Vacuolar alpha mannosidase; involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway (2, 3, 4)
Name Description Alpha-MannoSidase 1
Chromosomal Location
ChrVII:210416 to 213667 | ORF Map | GBrowse
Gbrowse
Genetic position: -98 cM
Gene Ontology Annotations All AMS1 GO evidence and references
  View Computational GO annotations for AMS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
Resources
24 total interaction(s) for 16 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Co-fractionation: 1
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,083
Molecular Weight (Da) 124,498
Isoelectric Point (pI) 7.22
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:210416 to 213667 | ORF Map | GBrowse
SGD ORF map
Genetic position: -98 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3252 210416..213667 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003124
References cited on this page View Complete Literature Guide for AMS1
1) Yoshihisa T and Anraku Y  (1989) Nucleotide sequence of AMS1, the structure gene of vacuolar alpha-mannosidase of Saccharomyces cerevisiae. Biochem Biophys Res Commun 163(2):908-15
2) Yoshihisa T and Anraku Y  (1990) A novel pathway of import of alpha-mannosidase, a marker enzyme of vacuolar membrane, in Saccharomyces cerevisiae. J Biol Chem 265(36):22418-25
3) Hutchins MU and Klionsky DJ  (2001) Vacuolar localization of oligomeric alpha-mannosidase requires the cytoplasm to vacuole targeting and autophagy pathway components in Saccharomyces cerevisiae. J Biol Chem 276(23):20491-8
4) Chantret I, et al.  (2003) Free-oligosaccharide control in the yeast Saccharomyces cerevisiae: roles for peptide:N-glycanase (Png1p) and vacuolar mannosidase (Ams1p). Biochem J 373(Pt 3):901-8