TIP20/YGL145W Summary Help

Standard Name TIP20 1
Systematic Name YGL145W
Alias TIP1 2
Feature Type ORF, Verified
Description Peripheral membrane protein required for COPI vesicle fusion to the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p (3, 4)
Name Description SEC20 (Twenty) Interacting Protein 2
Chromosomal Location
ChrVII:230243 to 232348 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All TIP20 GO evidence and references
  View Computational GO annotations for TIP20
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
conditional
null
repressible
unspecified
Large-scale survey
null
reduction of function
repressible
Resources
84 total interaction(s) for 27 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 24
  • Affinity Capture-Western: 15
  • Co-fractionation: 2
  • Reconstituted Complex: 6
  • Two-hybrid: 25

Genetic Interactions
  • Dosage Lethality: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
  • Synthetic Lethality: 9

Resources
Expression Summary
histogram
Resources
Length (a.a.) 701
Molecular Weight (Da) 81,166
Isoelectric Point (pI) 6.28
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:230243 to 232348 | ORF Map | GBrowse
This feature contains embedded feature(s): ARS701
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2106 230243..232348 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003113
References cited on this page View Complete Literature Guide for TIP20
1) Lewis MJ, et al.  (1997) A novel SNARE complex implicated in vesicle fusion with the endoplasmic reticulum. EMBO J 16(11):3017-24
2) Sweet DJ and Pelham HR  (1993) The TIP1 gene of Saccharomyces cerevisiae encodes an 80 kDa cytoplasmic protein that interacts with the cytoplasmic domain of Sec20p. EMBO J 12(7):2831-40
3) Kamena F and Spang A  (2004) Tip20p prohibits back-fusion of COPII vesicles with the endoplasmic reticulum. Science 304(5668):286-9
4) Tripathi A, et al.  (2009) Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nat Struct Mol Biol 16(2):114-23