CDC20/YGL116W Summary Help

Standard Name CDC20
Systematic Name YGL116W
Alias PAC5 1
Feature Type ORF, Verified
Description Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress (2 and see Summary Paragraph)
Name Description Cell Division Cycle 3
Chromosomal Location
ChrVII:289809 to 291641 | ORF Map | GBrowse
Gbrowse
Genetic position: -70 cM
Gene Ontology Annotations All CDC20 GO evidence and references
  View Computational GO annotations for CDC20
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulatory Role
Regulatory modules predicted: stressResponse (643, 547, 421, 409)
Mutant phenotypes All CDC20 Phenotype evidence and references
Classical genetics
conditional
repressible
Large-scale survey
conditional
null
overexpression
reduction of function
repressible
Resources
interactions All CDC20 Interaction evidence and references
363 total interaction(s) for 247 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 21
  • Affinity Capture-Western: 33
  • Biochemical Activity: 7
  • Co-fractionation: 4
  • Co-localization: 3
  • Co-purification: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 16
Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 6
  • Negative Genetic: 78
  • Phenotypic Enhancement: 7
  • Phenotypic Suppression: 10
  • Positive Genetic: 9
  • Synthetic Growth Defect: 97
  • Synthetic Lethality: 56
  • Synthetic Rescue: 8
Resources
Expression Summary
histogram
Resources
Protein Information All CDC20 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:289809 to 291641 | ORF Map | GBrowse
SGD ORF map
Genetic position: -70 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1833 289809..291641 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003084
SUMMARY PARAGRAPH for CDC20

CDC20 is an activator of the anaphase-promoting complex (APC), an E3 ubiquitin ligase in the ubiquitin-mediated proteolysis pathway (2). The APC ubiquitin ligase helps regulate the metaphase/anaphase transition and exit from mitosis/G1 entry through ubiquitination of various substrates. These include mitotic cyclins, the sister chromatid separation inhibitor Pds1p, the Kip1p and Cin8p motor proteins, Cdc5p, and the spindle disassembly factor, Ase1p (4, 5, 6, 7, 8, 9).

cdc20-1 mutants arrest in metaphase before the activation of APC-dependent proteolysis (10). Analysis of the mutants demonstrated that Cdc20p regulates the activity and substrate specificity of the APC (11). It serves as an activator of the APC and mediates ubiquitin-dependent protein degradation of Pds1p (6, 11), and the cyclins Clb5p and Clb3p (12) at the metaphase-to-anaphase transition of the cell cycle.

The timing of the association of Cdc20p with the APC is regulated. The levels of Cdc20p rise as cells enter mitosis and fall as cells exit mitosis, with the result that Cdc20 is bound to the APC only during M phase (and possibly during late G2) (13).

CDH1, another APC activator, is a homolog of CDC20 (11). CDC20 orthologs have also been identified in various species including worms, flies and humans (14, 2 , 11).

Last updated: 2005-05-11

References cited on this page View Complete Literature Guide for CDC20
1) Geiser JR, et al.  (1997) Saccharomyces cerevisiae genes required in the absence of the CIN8-encoded spindle motor act in functionally diverse mitotic pathways. Mol Biol Cell 8(6):1035-50
2) Zachariae W and Nasmyth K  (1999) Whose end is destruction: cell division and the anaphase-promoting complex. Genes Dev 13(16):2039-58
3) Hartwell LH, et al.  (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9
4) Juang YL, et al.  (1997) APC-mediated proteolysis of Ase1 and the morphogenesis of the mitotic spindle. Science 275(5304):1311-4
5) Cohen-Fix O, et al.  (1996) Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p. Genes Dev 10(24):3081-93
6) Shirayama M, et al.  (1998) The Polo-like kinase Cdc5p and the WD-repeat protein Cdc20p/fizzy are regulators and substrates of the anaphase promoting complex in Saccharomyces cerevisiae. EMBO J 17(5):1336-49
7) Gordon DM and Roof DM  (2001) Degradation of the kinesin Kip1p at anaphase onset is mediated by the anaphase-promoting complex and Cdc20p. Proc Natl Acad Sci U S A 98(22):12515-20
8) Hildebrandt ER and Hoyt MA  (2001) Cell cycle-dependent degradation of the Saccharomyces cerevisiae spindle motor Cin8p requires APC(Cdh1) and a bipartite destruction sequence. Mol Biol Cell 12(11):3402-16
9) Zachariae W and Nasmyth K  (1996) TPR proteins required for anaphase progression mediate ubiquitination of mitotic B-type cyclins in yeast. Mol Biol Cell 7(5):791-801
10) Sethi N, et al.  (1991) The CDC20 gene product of Saccharomyces cerevisiae, a beta-transducin homolog, is required for a subset of microtubule-dependent cellular processes. Mol Cell Biol 11(11):5592-602
11) Visintin R, et al.  (1997) CDC20 and CDH1: a family of substrate-specific activators of APC-dependent proteolysis. Science 278(5337):460-3
12) Alexandru G, et al.  (1999) Sister chromatid separation and chromosome re-duplication are regulated by different mechanisms in response to spindle damage. EMBO J 18(10):2707-21
13) Prinz S, et al.  (1998) The regulation of Cdc20 proteolysis reveals a role for APC components Cdc23 and Cdc27 during S phase and early mitosis. Curr Biol 8(13):750-60
14) Dawson IA, et al.  (1995) The Drosophila cell cycle gene fizzy is required for normal degradation of cyclins A and B during mitosis and has homology to the CDC20 gene of Saccharomyces cerevisiae. J Cell Biol 129(3):725-37