MLC1/YGL106W Summary Help

Standard Name MLC1 1
Systematic Name YGL106W
Feature Type ORF, Verified
Description Essential light chain for Myo1p; light chain for Myo2p; stabilizes Myo2p by binding to the neck region; interacts with Myo1p, Iqg1p, and Myo2p to coordinate formation and contraction of the actomyosin ring with targeted membrane deposition (1, 2, 3 and see Summary Paragraph)
Name Description Myosin Light Chain 1
Chromosomal Location
ChrVII:306560 to 307009 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MLC1 GO evidence and references
  View Computational GO annotations for MLC1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
repressible
Resources
128 total interaction(s) for 80 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 80
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 13
  • Co-crystal Structure: 3
  • Co-localization: 1
  • PCA: 3
  • Reconstituted Complex: 2
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Growth Defect: 5
  • Dosage Lethality: 1
  • Dosage Rescue: 7
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 149
Molecular Weight (Da) 16,444
Isoelectric Point (pI) 4.39
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:306560 to 307009 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..450 306560..307009 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003074
SUMMARY PARAGRAPH for MLC1

Mlc1p an essential light chain (ELC) for Myo1p, binding to its IQ1 motif, while Mlc2p is a regulatory light chain (RLC) for Myo1p (3, 4). Mlc1p is also a light chain for the type V myosin Myo2p (1) and the IQGAP-like protein, Iqg1p (5).

MLC1 is an essential gene and exhibits haploinsufficiency (1). Mlc1p localizes to the bud neck and its interactions with Myo1p and Iqg1p play a role in actin ring formation and promote targeted membrane deposition at the bud neck (3) during cytokinesis. Inviable mlc1 null mutant cells show a defect in cytokinesis, resulting in enlarged, multinucleate cells (1), a phenotype similar to cells deleted for IQG1 (6). Disruption of the Mlc1p-Myo1p or Mlc1p-Myo2p interaction does not prevent Mlc1p targeting to the bud neck. However, disrupting the Mlc1p-Iqg1p interaction by depleting Mlc1p prevents localization of Iqg1p to the bud neck which in turn prevents actin localization to the bud neck and actomyosin ring formation (4, 5). Thus, the Mlc1p-Iqg1p interaction plays a major role in cytokinesis. Mlc1p associates with secretory vesicles and forms a complex(es) with Sec4p and Myo2p and is required for vesicle targeting during cytokinesis (2).

Compared to the RLCs, the sequence of the ELCs are better conserved. Mlc1p is similar to S. pombe ELC (CDC4), D. discoideum ELC, chicken light chain (MLE1 gene) and Acanthamoeba myosin light chain (3 and references therein).

Last updated: 2007-02-15 Contact SGD

References cited on this page View Complete Literature Guide for MLC1
1) Stevens RC and Davis TN  (1998) Mlc1p is a light chain for the unconventional myosin Myo2p in Saccharomyces cerevisiae. J Cell Biol 142(3):711-22
2) Wagner W, et al.  (2002) Mlc1p promotes septum closure during cytokinesis via the IQ motifs of the vesicle motor Myo2p. EMBO J 21(23):6397-408
3) Luo J, et al.  (2004) Identification and functional analysis of the essential and regulatory light chains of the only type II myosin Myo1p in Saccharomyces cerevisiae. J Cell Biol 165(6):843-55
4) Boyne JR, et al.  (2000) Yeast myosin light chain, Mlc1p, interacts with both IQGAP and class II myosin to effect cytokinesis. J Cell Sci 113 Pt 24():4533-43
5) Shannon KB and Li R  (2000) A myosin light chain mediates the localization of the budding yeast IQGAP-like protein during contractile ring formation. Curr Biol 10(12):727-30
6) Epp JA and Chant J  (1997) An IQGAP-related protein controls actin-ring formation and cytokinesis in yeast. Curr Biol 7(12):921-9