LIF1/YGL090W Summary

Standard Name	LIF1 1
Systematic Name	YGL090W
Feature Type	ORF, Verified
Description	Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein (1, 2, 3 and see Summary Paragraph)
Name Description	Ligase Interacting Factor 1

Chromosomal Location	ChrVII:343319 to 344584 \| ORF Map \| GBrowse

Gene Ontology Annotations All LIF1 GO evidence and references

	View Computational GO annotations for LIF1
Molecular Function
Manually curated	chromatin binding (IDA)
Biological Process
Manually curated	double-strand break repair via nonhomologous end joining (IDA, IPI)
Cellular Component
Manually curated	DNA ligase IV complex (IPI) colocalizes_with nuclear chromatin (IDA) nucleus (IDA)

Mutant phenotypes All LIF1 Phenotype evidence and references

Classical genetics
null	X ray resistance: decreased cellular morphology: abnormal resistance to phleomycin: increased sporulation efficiency: decreased viable
Large-scale survey
null	competitive fitness: increased haploproficient Fus-Mid-GFP distribution: abnormal resistance to bleomycin: decreased resistance to caffeine: decreased viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All LIF1 Interaction evidence and references

	59 total interaction(s) for 28 unique genes/features.
Physical Interactions	Affinity Capture-MS: 8 Affinity Capture-RNA: 1 Affinity Capture-Western: 8 Co-crystal Structure: 1 Co-fractionation: 1 Co-purification: 2 Reconstituted Complex: 1 Two-hybrid: 22
Genetic Interactions	Negative Genetic: 5 Phenotypic Enhancement: 1 Phenotypic Suppression: 1 Positive Genetic: 2 Synthetic Growth Defect: 4 Synthetic Rescue: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All LIF1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVII:343319 to 344584 | |

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1266	343319..344584	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003058

SUMMARY PARAGRAPH for LIF1

Lif1p is required for targeting Dnl4p to double-strand break sites non-homologous end-joining (NHEJ) (1, 2). Lif1p assembles as part of the NHEJ machinery at sites of double-strand breaks (DSBs) (4, 5, 6). This machinery is required to align and process the DSBs in preparation for ligation by Dnl4p (reviewed in 7, 8). Lif1p interacts with the BRCT domain of Dnl4p (1, 9). This interaction is required for the stability and DNA ligase activity of Dnl4p (1, 2). The nuclear localization of Lif1p may be regulated by Nej1p (10). lif1 mutants undergo meiosis and sporulation less efficiently than wild type cells (1).

Lif1p is widely conserved and is known as XRCC4 in humans (1, 9). Lif1p has sequence similarity with human XRCC4 in the region that interacts with the BRCT domain of Dnl4p (1).

Last updated: 2007-07-12

References cited on this page View Complete Literature Guide for LIF1

1)	Herrmann G, et al. (1998) Saccharomyces cerevisiae LIF1: a function involved in DNA double-strand break repair related to mammalian XRCC4. EMBO J 17(14):4188-98
2)	Teo SH and Jackson SP (2000) Lif1p targets the DNA ligase Lig4p to sites of DNA double-strand breaks. Curr Biol 10(3):165-8
3)	Deshpande RA and Wilson TE (2007) Modes of interaction among yeast Nej1, Lif1 and Dnl4 proteins and comparison to human XLF, XRCC4 and Lig4. DNA Repair (Amst) 6(10):1507-16
4)	Chen L, et al. (2001) Promotion of Dnl4-catalyzed DNA end-joining by the Rad50/Mre11/Xrs2 and Hdf1/Hdf2 complexes. Mol Cell 8(5):1105-15
5)	Tseng HM and Tomkinson AE (2004) Processing and joining of DNA ends coordinated by interactions among Dnl4/Lif1, Pol4, and FEN-1. J Biol Chem 279(46):47580-8
6)	Zhang Y, et al. (2007) Role of Dnl4-Lif1 in nonhomologous end-joining repair complex assembly and suppression of homologous recombination. Nat Struct Mol Biol 14(7):639-46
7)	Daley JM, et al. (2005) Nonhomologous end joining in yeast. Annu Rev Genet 39():431-51
8)	Hefferin ML and Tomkinson AE (2005) Mechanism of DNA double-strand break repair by non-homologous end joining. DNA Repair (Amst) 4(6):639-48
9)	Dore AS, et al. (2006) Structure of an Xrcc4-DNA ligase IV yeast ortholog complex reveals a novel BRCT interaction mode. DNA Repair (Amst) 5(3):362-8
10)	Valencia M, et al. (2001) NEJ1 controls non-homologous end joining in Saccharomyces cerevisiae. Nature 414(6864):666-9