PKP2/YGL059W Summary Help

Standard Name PKP2 1
Systematic Name YGL059W
Feature Type ORF, Verified
Description Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress (1, 2, 3, 4)
Name Description Protein Kinase of PDH 1
Chromosomal Location
ChrVII:392223 to 393698 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PKP2 GO evidence and references
  View Computational GO annotations for PKP2
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
121 total interaction(s) for 117 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Biochemical Activity: 92

Genetic Interactions
  • Negative Genetic: 15
  • Phenotypic Enhancement: 1
  • Positive Genetic: 5
  • Synthetic Growth Defect: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 491
Molecular Weight (Da) 57,294
Isoelectric Point (pI) 8.17
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:392223 to 393698 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1476 392223..393698 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003027
References cited on this page View Complete Literature Guide for PKP2
1) Gey U, et al.  (2008) Yeast pyruvate dehydrogenase complex is regulated by a concerted activity of two kinases and two phosphatases. J Biol Chem 283(15):9759-67
2) Zhu H, et al.  (2000) Analysis of yeast protein kinases using protein chips. Nat Genet 26(3):283-9
3) Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76