CNN1/YFR046C Summary Help

Standard Name CNN1 1
Systematic Name YFR046C
Feature Type ORF, Verified
Description Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T (1, 2, 3, 4, 5, 6)
Name Description Co-purified with NNf1p 1
Chromosomal Location
ChrVI:244159 to 243074 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All CNN1 GO evidence and references
  View Computational GO annotations for CNN1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
67 total interaction(s) for 39 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 28
  • Affinity Capture-Western: 1
  • Biochemical Activity: 7
  • Co-crystal Structure: 1
  • Co-purification: 4
  • Reconstituted Complex: 3
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 6
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 361
Molecular Weight (Da) 41,313
Isoelectric Point (pI) 4.43
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVI:244159 to 243074 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1086 244159..243074 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001942
References cited on this page View Complete Literature Guide for CNN1
1) De Wulf P, et al.  (2003) Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes. Genes Dev 17(23):2902-21
2) Cho RJ, et al.  (1998) Parallel analysis of genetic selections using whole genome oligonucleotide arrays. Proc Natl Acad Sci U S A 95(7):3752-7
3) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
4) Loog M and Morgan DO  (2005) Cyclin specificity in the phosphorylation of cyclin-dependent kinase substrates. Nature 434(7029):104-8
5) Schleiffer A, et al.  (2012) CENP-T proteins are conserved centromere receptors of the Ndc80 complex. Nat Cell Biol 14(6):604-13
6) Bock LJ, et al.  (2012) Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore. Nat Cell Biol 14(6):614-24