SAP155/YFR040W Summary Help

Standard Name SAP155 1
Systematic Name YFR040W
Feature Type ORF, Verified
Description Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress; SAP155 has a paralog, SAP4, that arose from the whole genome duplication (1, 2, 3)
Name Description Sit4 Associated Protein 1
Chromosomal Location
ChrVI:234242 to 237250 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SAP155 GO evidence and references
  View Computational GO annotations for SAP155
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
unspecified
Resources
203 total interaction(s) for 150 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 27
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 4
  • PCA: 6
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 3
  • Dosage Rescue: 10
  • Negative Genetic: 97
  • Phenotypic Enhancement: 1
  • Positive Genetic: 20
  • Synthetic Growth Defect: 17
  • Synthetic Lethality: 5
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,002
Molecular Weight (Da) 115,002
Isoelectric Point (pI) 4.31
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVI:234242 to 237250 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3009 234242..237250 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001936
References cited on this page View Complete Literature Guide for SAP155
1) Luke MM, et al.  (1996) The SAP, a new family of proteins, associate and function positively with the SIT4 phosphatase. Mol Cell Biol 16(6):2744-55
2) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76