LSB3/YFR024C-A Summary Help

Standard Name LSB3 1
Systematic Name YFR024C-A
Alias YFR024C
Feature Type ORF, Verified
Description Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication (1, 2, 3)
Name Description Las Seventeen Binding protein 1
Chromosomal Location
ChrVI:203433 to 201960 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All LSB3 GO evidence and references
  View Computational GO annotations for LSB3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
170 total interaction(s) for 125 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 40
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Biochemical Activity: 4
  • PCA: 9
  • Protein-peptide: 8
  • Reconstituted Complex: 2
  • Two-hybrid: 85

Genetic Interactions
  • Negative Genetic: 12
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 459
Molecular Weight (Da) 49,344
Isoelectric Point (pI) 8.34
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVI:203433 to 201960 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
This feature contains embedded feature(s): YFR024C
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..47 203433..203387 2011-02-03 1996-07-31
Intron 48..141 203386..203293 2011-02-03 2008-06-02
CDS 142..1474 203292..201960 2011-02-03 2008-06-02
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002968
References cited on this page View Complete Literature Guide for LSB3
1) Madania A, et al.  (1999) The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex. Mol Biol Cell 10(10):3521-38
2) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76