UBP6/YFR010W Summary Help

Standard Name UBP6 1
Systematic Name YFR010W
Feature Type ORF, Verified
Description Ubiquitin-specific protease; situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; negatively regulates degradation of ubiquitinated proteins by the proteasome; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance (1, 2, 3, 4, 5, 6, 7)
Name Description UBiquitin-specific Protease 1
Chromosomal Location
ChrVI:165067 to 166566 | ORF Map | GBrowse
Gene Ontology Annotations All UBP6 GO evidence and references
  View Computational GO annotations for UBP6
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
Large-scale survey
639 total interaction(s) for 359 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 113
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 6
  • Biochemical Activity: 9
  • Co-purification: 5
  • PCA: 4
  • Reconstituted Complex: 5
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 360
  • Phenotypic Enhancement: 9
  • Phenotypic Suppression: 3
  • Positive Genetic: 39
  • Synthetic Growth Defect: 38
  • Synthetic Lethality: 25
  • Synthetic Rescue: 19

Expression Summary
Length (a.a.) 499
Molecular Weight (Da) 57,110
Isoelectric Point (pI) 7.14
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVI:165067 to 166566 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1500 165067..166566 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001906
References cited on this page View Complete Literature Guide for UBP6
1) Park KC, et al.  (1997) Purification and characterization of UBP6, a new ubiquitin-specific protease in Saccharomyces cerevisiae. Arch Biochem Biophys 347(1):78-84
2) Hanna J, et al.  (2003) Ubiquitin depletion as a key mediator of toxicity by translational inhibitors. Mol Cell Biol 23(24):9251-61
3) Chernova TA, et al.  (2003) Pleiotropic effects of Ubp6 loss on drug sensitivities and yeast prion are due to depletion of the free ubiquitin pool. J Biol Chem 278(52):52102-15
4) Guterman A and Glickman MH  (2004) Complementary roles for Rpn11 and Ubp6 in deubiquitination and proteolysis by the proteasome. J Biol Chem 279(3):1729-38
5) Hanna J, et al.  (2006) Deubiquitinating enzyme Ubp6 functions noncatalytically to delay proteasomal degradation. Cell 127(1):99-111
6) Crosas B, et al.  (2006) Ubiquitin chains are remodeled at the proteasome by opposing ubiquitin ligase and deubiquitinating activities. Cell 127(7):1401-13
7) Torres EM, et al.  (2010) Identification of aneuploidy-tolerating mutations. Cell 143(1):71-83