Standard Name	CDC4
Systematic Name	YFL009W
Feature Type	ORF, Verified
Description	F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p; Cdc4p interacts with G1 cyclin Cln3p, and Cdc4p and Grr1p redundantly target Cln3p for degradation. (1, 2, 3, 4)
Name Description	Cell Division Cycle 5

Chromosomal Location	ChrVI:116145 to 118484 | ORF Map | GBrowse
	Genetic position: -9 cM

	View Computational GO annotations for CDC4
Molecular Function
Manually curated	ubiquitin binding (IDA) contributes_to ubiquitin-protein ligase activity (IDA)
Biological Process
Manually curated	G1/S transition of mitotic cell cycle (IMP) G2/M transition of mitotic cell cycle (IGI, IMP) meiosis (IMP) protein ubiquitination involved in ubiquitin-dependent protein catabolic process (IDA) SCF-dependent proteasomal ubiquitin-dependent protein catabolic process (IDA)
Cellular Component
Manually curated	nuclear matrix (IDA) nuclear SCF ubiquitin ligase complex (IDA) nucleus (IDA) SCF ubiquitin ligase complex (IDA, IPI)

Classical genetics
conditional	cell cycle progression: arrested cell cycle progression through the G1/S phase transition: arrested cell cycle progression through the G2/M phase transition: arrested heat sensitivity: increased Gpa1p accumulation: increased Cln2p-13Myc accumulation: increased
repressible	Fus3p modification: decreased
Large-scale survey
conditional	colony sectoring: increased heat sensitivity: increased
null	inviable
reduction of function	competitive fitness: decreased
repressible	cell cycle progression in G1 phase: abnormal
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	205 total interaction(s) for 117 unique genes/features.
Physical Interactions	Affinity Capture-MS: 8 Affinity Capture-Western: 31 Biochemical Activity: 8 Co-crystal Structure: 2 Co-fractionation: 1 Co-localization: 1 Co-purification: 2 Protein-peptide: 1 Reconstituted Complex: 23 Two-hybrid: 22
Genetic Interactions	Dosage Lethality: 5 Dosage Rescue: 4 Negative Genetic: 56 Phenotypic Enhancement: 1 Phenotypic Suppression: 9 Positive Genetic: 2 Synthetic Growth Defect: 8 Synthetic Lethality: 11 Synthetic Rescue: 10
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrVI:116145 to 118484 | ORF Map | GBrowse
	Genetic position: -9 cM
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2340 116145..118484 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001885