SMC1/YFL008W Summary Help

Standard Name SMC1 1
Systematic Name YFL008W
Alias CHL10
Feature Type ORF, Verified
Description Subunit of the multiprotein cohesin complex; essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure (2, 3, 4 and see Summary Paragraph)
Name Description Stability of MiniChromosomes 3, 5
Chromosomal Location
ChrVI:119429 to 123106 | ORF Map | GBrowse
Gbrowse
Genetic position: -10 cM
Gene Ontology Annotations All SMC1 GO evidence and references
  View Computational GO annotations for SMC1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
conditional
null
Large-scale survey
conditional
null
reduction of function
Resources
256 total interaction(s) for 159 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 42
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 23
  • Co-localization: 1
  • Co-purification: 4
  • FRET: 4
  • Reconstituted Complex: 6
  • Two-hybrid: 29

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 2
  • Negative Genetic: 117
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 10
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 9

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,225
Molecular Weight (Da) 141,279
Isoelectric Point (pI) 6.41
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVI:119429 to 123106 | ORF Map | GBrowse
SGD ORF map
Genetic position: -10 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3678 119429..123106 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001886
SUMMARY PARAGRAPH for SMC1

SMC1 is an essential gene that encodes a member of a ubiquitous family of chromosome-associated ATPases (3, 2, 6, 7). SMC proteins are found in eukaryotes, prokaryotes, and archaea, and appear to play roles in chromosome dynamics (6, 7). In eukaryotes, SMC proteins form two kinds of heterodimers, corresponding to Smc1p-Smc3p and Smc2p-Smc4p in yeast (6, 7). The Smc1p-Smc3p heterodimer interacts with additional proteins, including Mcd1p and Irr1p, to form the yeast cohesin complex, which is required for sister chromatid cohesion in mitosis and meiosis (8, 9, 10, 6, 7). The yeast cohesin complex associates with centromeres and other discrete sites along chromosome arms prior to metaphase; the association with centromeres requires the centromere protein Mif2p, the centromere binding complex CBF3, and Cse4p (11, 12). Scc2p is not a stoichiometric cohesin subunit, but is required for the cohesin complex to associate with chromosomes (9).

Conditional lethal mutations in SMC1 cause increased chromosome loss and premature dissociation of sister chromatids (5, 3, 13, 14). The smc1-2 mutation can be suppressed by overexpression of TID3, which encodes a component of the spindle pole body; Tid3p also interacts physically with Smc1p (15).

Cohesin complexes that include homologs of Smc1p and Smc3p have been identified in other eukaryotes, including Xenopus, C. elegans, S. pombe, and human (6). SMC protein homodimers have been found in prokaryotes (7).

Last updated: 2000-05-03 Contact SGD

References cited on this page View Complete Literature Guide for SMC1
1) Larionov, V.  (1989) Personal Communication, Mortimer Map Edition 10
2) Akhmedov AT, et al.  (1998) Structural maintenance of chromosomes protein C-terminal domains bind preferentially to DNA with secondary structure. J Biol Chem 273(37):24088-94
3) Strunnikov AV, et al.  (1993) SMC1: an essential yeast gene encoding a putative head-rod-tail protein is required for nuclear division and defines a new ubiquitous protein family. J Cell Biol 123(6 Pt 2):1635-48
4) Schar P, et al.  (2004) SMC1 coordinates DNA double-strand break repair pathways. Nucleic Acids Res 32(13):3921-9
5) Larionov VL, et al.  (1985) A mutant of Saccharomyces cerevisiae with impaired maintenance of centromeric plasmids. Curr Genet 10(1):15-20
6) Hirano T  (1999) SMC-mediated chromosome mechanics: a conserved scheme from bacteria to vertebrates? Genes Dev 13(1):11-9
7) Strunnikov AV and Jessberger R  (1999) Structural maintenance of chromosomes (SMC) proteins: conserved molecular properties for multiple biological functions. Eur J Biochem 263(1):6-13
8) Guacci V, et al.  (1997) A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S. cerevisiae. Cell 91(1):47-57
9) Toth A, et al.  (1999) Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication. Genes Dev 13(3):320-33
10) Klein F, et al.  (1999) A central role for cohesins in sister chromatid cohesion, formation of axial elements, and recombination during yeast meiosis. Cell 98(1):91-103
11) Tanaka T, et al.  (1999) Identification of cohesin association sites at centromeres and along chromosome arms. Cell 98(6):847-58
12) Blat Y and Kleckner N  (1999) Cohesins bind to preferential sites along yeast chromosome III, with differential regulation along arms versus the centric region. Cell 98(2):249-59
13) Michaelis C, et al.  (1997) Cohesins: chromosomal proteins that prevent premature separation of sister chromatids. Cell 91(1):35-45
14) Uhlmann F and Nasmyth K  (1998) Cohesion between sister chromatids must be established during DNA replication. Curr Biol 8(20):1095-101
15) Zheng L, et al.  (1999) Hec1p, an evolutionarily conserved coiled-coil protein, modulates chromosome segregation through interaction with SMC proteins. Mol Cell Biol 19(8):5417-28