DEG1/YFL001W Summary Help

Standard Name DEG1 1
Systematic Name YFL001W
Alias PUS3 , HRM3 2
Feature Type ORF, Verified
Description tRNA:pseudouridine synthase; introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability (1, 3, 4)
Name Description DEpressed Growth rate 1
Chromosomal Location
ChrVI:147131 to 148459 | ORF Map | GBrowse
Gbrowse
Genetic position: -1 cM
Gene Ontology Annotations All DEG1 GO evidence and references
  View Computational GO annotations for DEG1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
overexpression
Resources
439 total interaction(s) for 300 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 6
  • Reconstituted Complex: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 300
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 4
  • Positive Genetic: 104
  • Synthetic Growth Defect: 12
  • Synthetic Lethality: 3
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 442
Molecular Weight (Da) 50,888
Isoelectric Point (pI) 10.02
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVI:147131 to 148459 | ORF Map | GBrowse
SGD ORF map
Genetic position: -1 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1329 147131..148459 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001895
References cited on this page View Complete Literature Guide for DEG1
1) Carbone ML, et al.  (1991) A gene tightly linked to CEN6 is important for growth of Saccharomyces cerevisiae. Curr Genet 19(1):1-8
2) Hepfer CE, et al.  (2005) DEG1, encoding the tRNA:pseudouridine synthase Pus3p, impacts HOT1-stimulated recombination in Saccharomyces cerevisiae. Mol Genet Genomics 274(5):528-38
3) Lecointe F, et al.  (1998) Characterization of yeast protein Deg1 as pseudouridine synthase (Pus3) catalyzing the formation of psi 38 and psi 39 in tRNA anticodon loop. J Biol Chem 273(3):1316-23
4) Lecointe F, et al.  (2002) Lack of pseudouridine 38/39 in the anticodon arm of yeast cytoplasmic tRNA decreases in vivo recoding efficiency. J Biol Chem 277(34):30445-53