PDA1/YER178W Summary

Standard Name	PDA1
Systematic Name	YER178W
Feature Type	ORF, Verified
Description	E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose (1, 2, 3, 4 and see Summary Paragraph)
Name Description	Pyruvate Dehydrogenase Alpha 1

Chromosomal Location	ChrV:546817 to 548079 \| ORF Map \| GBrowse

	Genetic position: 143 cM

Gene Ontology Annotations All PDA1 GO evidence and references

	View Computational GO annotations for PDA1
Molecular Function
Manually curated	pyruvate dehydrogenase (acetyl-transferring) activity (IMP)
Biological Process
Manually curated	acetyl-CoA biosynthetic process from pyruvate (IDA)
Cellular Component
Manually curated	mitochondrial nucleoid (IDA) mitochondrial pyruvate dehydrogenase complex (IDA) mitochondrion (IDA)
High-throughput	mitochondrion (IDA)

Pathways	pyruvate dehydrogenase complex

Mutant phenotypes All PDA1 Phenotype evidence and references

Classical genetics
null	resistance to sirolimus: decreased
Large-scale survey
null	chitin deposition: normal competitive fitness: decreased heat sensitivity: increased resistance to camptothecin: decreased resistance to fenpropimorph: increased resistance to cycloheximide: decreased resistance to amitrole: decreased resistance to methyl methanesulfonate: decreased resistance to tunicamycin: decreased resistance to edelfosine: decreased resistance to fluconazole: increased respiratory growth: decreased rate starvation resistance: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All PDA1 Interaction evidence and references

	310 total interaction(s) for 217 unique genes/features.
Physical Interactions	Affinity Capture-MS: 29 Affinity Capture-RNA: 3 Affinity Capture-Western: 1 Co-fractionation: 2 Two-hybrid: 1
Genetic Interactions	Negative Genetic: 226 Positive Genetic: 31 Synthetic Growth Defect: 8 Synthetic Lethality: 6 Synthetic Rescue: 3
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All PDA1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrV:546817 to 548079 | |

: 143 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 2003-09-22

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1263	546817..548079	2011-02-03	2003-09-22

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000980

SUMMARY PARAGRAPH for PDA1

PDA1 encodes the alpha subunit of pyruvate dehydrogenase (5, 1). Pyruvate dehydrogenase, consisting of alpha (Pda1p) and beta (Pdb1p) subunits, is the E1 component of the large multienzyme pyruvate dehydrogenase complex (3). This mitochondrial complex consists of an E2 (Lat1p) core that binds E1 (Pda1p and Pdb1p ), E3 (Lpd1p), and Protein X (Pdx1p) components (3). E1 (pyruvate dehydrogenase), E2 (dihydrolipoamide acetyltransferase), and E3 (dihydrolipoamide dehydrogenase) act in concert to convert pyruvate into acetyl-CoA (3). Cells lacking PDA1 are viable but lack pyruvate dehydrogenase activity, show slower growth on glucose, and exhibit increased formation of petites that lack mitochondrial DNA (rho0) (1, 6). The yeast PDA1 gene shows strong similarity to human PDHA1, mutations in which are responsible for most cases of pyruvate dehydrogenase deficiency (OMIM) (7).

Last updated: 2005-07-01

References cited on this page View Complete Literature Guide for PDA1

1)	Steensma HY, et al. (1990) Molecular cloning of the gene for the E1 alpha subunit of the pyruvate dehydrogenase complex from Saccharomyces cerevisiae. Eur J Biochem 191(3):769-74
2)	Sierkstra LN, et al. (1992) Analysis of transcription and translation of glycolytic enzymes in glucose-limited continuous cultures of Saccharomyces cerevisiae. J Gen Microbiol 138(12):2559-66
3)	Pronk JT, et al. (1996) Pyruvate metabolism in Saccharomyces cerevisiae. Yeast 12(16):1607-33
4)	Reinders J, et al. (2007) Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol Cell Proteomics 6(11):1896-906
5)	Behal RH, et al. (1989) Nucleotide and deduced amino acid sequence of the alpha subunit of yeast pyruvate dehydrogenase. Biochem Biophys Res Commun 164(2):941-6
6)	Wenzel TJ, et al. (1992) Characterization of Saccharomyces cerevisiae mutants lacking the E1 alpha subunit of the pyruvate dehydrogenase complex. Eur J Biochem 209(2):697-705
7)	Foury F (1997) Human genetic diseases: a cross-talk between man and yeast. Gene 195(1):1-10