PDA1/YER178W Summary Help

Standard Name PDA1 1
Systematic Name YER178W
Feature Type ORF, Verified
Description E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex; catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description Pyruvate Dehydrogenase Alpha 1
Chromosomal Location
ChrV:546817 to 548079 | ORF Map | GBrowse
Genetic position: 143 cM
Gene Ontology Annotations All PDA1 GO evidence and references
  View Computational GO annotations for PDA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 12 genes
Classical genetics
Large-scale survey
313 total interaction(s) for 216 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 30
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 1
  • Co-fractionation: 2
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 227
  • Positive Genetic: 31
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 6
  • Synthetic Rescue: 3

Expression Summary
Length (a.a.) 420
Molecular Weight (Da) 46,343
Isoelectric Point (pI) 8.13
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrV:546817 to 548079 | ORF Map | GBrowse
Genetic position: 143 cM
Last Update Coordinates: 2011-02-03 | Sequence: 2003-09-22
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1263 546817..548079 2011-02-03 2003-09-22
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000980

PDA1 encodes the alpha subunit of pyruvate dehydrogenase (6, 1). Pyruvate dehydrogenase, consisting of alpha (Pda1p) and beta (Pdb1p) subunits, is the E1 component of the large multienzyme pyruvate dehydrogenase complex (3). This mitochondrial complex consists of an E2 (Lat1p) core that binds E1 (Pda1p and Pdb1p ), E3 (Lpd1p), and Protein X (Pdx1p) components (3). E1 (pyruvate dehydrogenase), E2 (dihydrolipoamide acetyltransferase), and E3 (dihydrolipoamide dehydrogenase) act in concert to convert pyruvate into acetyl-CoA (3). Cells lacking PDA1 are viable but lack pyruvate dehydrogenase activity, show slower growth on glucose, and exhibit increased formation of petites that lack mitochondrial DNA (rho0) (1, 7). The yeast PDA1 gene shows strong similarity to human PDHA1, mutations in which are responsible for most cases of pyruvate dehydrogenase deficiency (OMIM) (8).

Last updated: 2005-07-01 Contact SGD

References cited on this page View Complete Literature Guide for PDA1
1) Steensma HY, et al.  (1990) Molecular cloning of the gene for the E1 alpha subunit of the pyruvate dehydrogenase complex from Saccharomyces cerevisiae. Eur J Biochem 191(3):769-74
2) Sierkstra LN, et al.  (1992) Analysis of transcription and translation of glycolytic enzymes in glucose-limited continuous cultures of Saccharomyces cerevisiae. J Gen Microbiol 138(12):2559-66
3) Pronk JT, et al.  (1996) Pyruvate metabolism in Saccharomyces cerevisiae. Yeast 12(16):1607-33
4) Reinders J, et al.  (2007) Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol Cell Proteomics 6(11):1896-906
5) Cohen Y, et al.  (2014) Peroxisomes are juxtaposed to strategic sites on mitochondria. Mol Biosyst 10(7):1742-8
6) Behal RH, et al.  (1989) Nucleotide and deduced amino acid sequence of the alpha subunit of yeast pyruvate dehydrogenase. Biochem Biophys Res Commun 164(2):941-6
7) Wenzel TJ, et al.  (1992) Characterization of Saccharomyces cerevisiae mutants lacking the E1 alpha subunit of the pyruvate dehydrogenase complex. Eur J Biochem 209(2):697-705
8) Foury F  (1997) Human genetic diseases: a cross-talk between man and yeast. Gene 195(1):1-10