BRR2/YER172C Summary Help

BRR2 BASIC INFORMATION

Standard Name BRR2 1
Systematic Name YER172C
Alias RSS1 2 , SLT22 3 , 4 , SNU246 5 , PRP44
Feature Type ORF, Verified
Description RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD (1, 4, 5, 6)
Name Description Bad Response to Refrigeration 1
GO Annotations All BRR2 GO evidence and references
    View Computational GO annotations for BRR2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All BRR2 Phenotype details and references
Classical genetics
null
repressible
Large-scale survey
null
Interactions BRR2 All interactions details and references
297 total interaction(s) for 88 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 173
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 36
  • Co-purification: 50
  • Protein-peptide: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 26
Genetic Interactions
  • Phenotypic Suppression: 2
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 4
  • Synthetic Rescue: 1
Sequence Information
ChrV:536016 to 529525 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..6492 536016..529525 1996-07-31 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000000974

BRR2 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for BRR2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for BRR2]

1) Noble SM and Guthrie C  (1996) Identification of novel genes required for yeast pre-mRNA splicing by means of cold-sensitive mutations. Genetics 143(1):67-80
2) Lin J and Rossi JJ  (1996) Identification and characterization of yeast mutants that overcome an experimentally introduced block to splicing at the 3' splice site. RNA 2(8):835-48
3) Xu D, et al.  (1998) Synthetic lethality of yeast slt mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing. Mol Cell Biol 18(4):2055-66
4) Xu D, et al.  (1996) An RNA-dependent ATPase associated with U2/U6 snRNAs in pre-mRNA splicing. Nature 381(6584):709-13
5) Lauber J, et al.  (1996) The HeLa 200 kDa U5 snRNP-specific protein and its homologue in Saccharomyces cerevisiae are members of the DEXH-box protein family of putative RNA helicases. EMBO J 15(15):4001-15
6) Raghunathan PL and Guthrie C  (1998) RNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2. Curr Biol 8(15):847-55