BRR2/YER172C Summary Help

Standard Name BRR2 1
Systematic Name YER172C
Alias RSS1 2 , SLT22 3 , 4 , SNU246 5 , PRP44
Feature Type ORF, Verified
Description RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD (1, 4, 5, 6)
Name Description Bad Response to Refrigeration 1
Chromosomal Location
ChrV:536021 to 529530 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All BRR2 GO evidence and references
  View Computational GO annotations for BRR2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
Large-scale survey
341 total interaction(s) for 115 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 193
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 38
  • Co-crystal Structure: 1
  • Co-fractionation: 2
  • Co-purification: 50
  • Protein-peptide: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 27

Genetic Interactions
  • Negative Genetic: 12
  • Positive Genetic: 2
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 6
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 2,163
Molecular Weight (Da) 246,183
Isoelectric Point (pI) 5.28
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrV:536021 to 529530 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..6492 536021..529530 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000974
References cited on this page View Complete Literature Guide for BRR2
1) Noble SM and Guthrie C  (1996) Identification of novel genes required for yeast pre-mRNA splicing by means of cold-sensitive mutations. Genetics 143(1):67-80
2) Lin J and Rossi JJ  (1996) Identification and characterization of yeast mutants that overcome an experimentally introduced block to splicing at the 3' splice site. RNA 2(8):835-48
3) Xu D, et al.  (1998) Synthetic lethality of yeast slt mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing. Mol Cell Biol 18(4):2055-66
4) Xu D, et al.  (1996) An RNA-dependent ATPase associated with U2/U6 snRNAs in pre-mRNA splicing. Nature 381(6584):709-13
5) Lauber J, et al.  (1996) The HeLa 200 kDa U5 snRNP-specific protein and its homologue in Saccharomyces cerevisiae are members of the DEXH-box protein family of putative RNA helicases. EMBO J 15(15):4001-15
6) Raghunathan PL and Guthrie C  (1998) RNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2. Curr Biol 8(15):847-55