PEA2/YER149C Summary Help

Standard Name PEA2 1
Systematic Name YER149C
Alias DFG9 2 , PPF2 3 , 4
Feature Type ORF, Verified
Description Coiled-coil 12S polarisome subunit; required for polarity establishment, apical bud growth, shmoo formation during mating, and filamentous differentiation; involved in the localization of Bni1p at sites of polarized growth, thereby controlling the polarized assembly of actin cables; role in apical growth affects diploid-specific bipolar bud site selection; retains Slt2p at the bud tip to regulate ER inheritance; role in low affinity Ca2+ influx and cell fusion during mating (1, 3, 5, 6, 7)
Name Description PEAnut shmoo mutant 1
Chromosomal Location
ChrV:467470 to 466208 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PEA2 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
reduction of function
unspecified
Large-scale survey
null
Resources
94 total interaction(s) for 70 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-Western: 2
  • Reconstituted Complex: 2
  • Two-hybrid: 19

Genetic Interactions
  • Negative Genetic: 36
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 3
  • Positive Genetic: 1
  • Synthetic Growth Defect: 9
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 15
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 420
Molecular Weight (Da) 48,164
Isoelectric Point (pI) 5.37
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:467470 to 466208 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1263 467470..466208 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000951
References cited on this page View Complete Literature Guide for PEA2
1) Chenevert J, et al.  (1994) Identification of genes required for normal pheromone-induced cell polarization in Saccharomyces cerevisiae. Genetics 136(4):1287-96
2) Mosch HU and Fink GR  (1997) Dissection of filamentous growth by transposon mutagenesis in Saccharomyces cerevisiae. Genetics 145(3):671-84
3) Valtz N and Herskowitz I  (1996) Pea2 protein of yeast is localized to sites of polarized growth and is required for efficient mating and bipolar budding. J Cell Biol 135(3):725-39
4) Yorihuzi T and Ohsumi Y  (1994) Saccharomyces cerevisiae MATa mutant cells defective in pointed projection formation in response to alpha-factor at high concentrations. Yeast 10(5):579-94
5) Sheu YJ, et al.  (2000) Polarized growth controls cell shape and bipolar bud site selection in Saccharomyces cerevisiae. Mol Cell Biol 20(14):5235-47
6) Sagot I, et al.  (2002) Yeast formins regulate cell polarity by controlling the assembly of actin cables. Nat Cell Biol 4(1):42-50
7) Li X, et al.  (2013) Different polarisome components play distinct roles in Slt2p-regulated cortical ER inheritance in Saccharomyces cerevisiae. Mol Biol Cell 24(19):3145-54