Standard Name	YCK3
Systematic Name	YER123W
Alias	CKI3
Feature Type	ORF, Verified
Description	Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway (1, 2, 3, 4)
Name Description	Yeast Casein Kinase 1

Chromosomal Location	ChrV:404813 to 406387 | ORF Map | GBrowse

	View Computational GO annotations for YCK3
Molecular Function
Manually curated	protein serine/threonine kinase activity (ISS)
High-throughput	protein kinase activity (IDA)
Biological Process
Manually curated	protein phosphorylation (ISS) vesicle-mediated transport (IMP)
High-throughput	protein phosphorylation (IDA)
Cellular Component
Manually curated	fungal-type vacuole (IDA) fungal-type vacuole membrane (IDA) nucleus (IDA) plasma membrane (IDA)

Classical genetics
null	endomembrane system morphology: abnormal metal resistance: decreased GFP-Vps41p distribution: abnormal toxin resistance: increased vacuolar morphology: abnormal
overexpression	vacuolar morphology: abnormal
Large-scale survey
null	competitive fitness: decreased dessication resistance: decreased haploinsufficient hyperosmotic stress resistance: decreased metal resistance: decreased Pho8p (ALP) modification: decreased resistance to neomycin: decreased resistance to cycloheximide: decreased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to methyl methanesulfonate: decreased resistance to nickel sulfate: decreased resistance to propolis extract: decreased resistance to amitrole: decreased resistance to calcium dichloride: decreased sporulation: normal starvation resistance: decreased vacuolar morphology: abnormal vegetative growth: abnormal viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	105 total interaction(s) for 98 unique genes/features.
Physical Interactions	Affinity Capture-MS: 6 Affinity Capture-RNA: 1 Biochemical Activity: 4 Co-fractionation: 1 Reconstituted Complex: 4
Genetic Interactions	Dosage Growth Defect: 2 Dosage Rescue: 1 Negative Genetic: 66 Phenotypic Enhancement: 2 Positive Genetic: 9 Synthetic Growth Defect: 5 Synthetic Haploinsufficiency: 1 Synthetic Rescue: 3
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrV:404813 to 406387 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 2000-03-16
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1575 404813..406387 2011-02-03 2000-03-16
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000925