SWI4/YER111C Summary Help

Standard Name SWI4 1
Systematic Name YER111C
Alias ART1
Feature Type ORF, Verified
Description DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; Slt2p-independent regulator of cold growth; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p (2, 3, 4, 5, 6, 7, 8, 9, 10)
Name Description SWItching deficient 1
Chromosomal Location
ChrV:385876 to 382595 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SWI4 GO evidence and references
  View Computational GO annotations for SWI4
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulatory Role
Binding motifs Predicted SWI4 Binding Site Locations
    Regulatory modules predicted: cellcycle (257)
    Resources
    Mutant phenotypes All SWI4 Phenotype evidence and references
    Classical genetics
    null
    overexpression
    unspecified
    Large-scale survey
    null
    overexpression
    Resources
    interactions All SWI4 Interaction evidence and references
    800 total interaction(s) for 419 unique genes/features.
    Physical Interactions
    • Affinity Capture-MS: 15
    • Affinity Capture-RNA: 2
    • Affinity Capture-Western: 23
    • Biochemical Activity: 2
    • Co-purification: 1
    • PCA: 2
    • Reconstituted Complex: 8
    • Two-hybrid: 2
    Genetic Interactions
    • Dosage Lethality: 2
    • Dosage Rescue: 32
    • Negative Genetic: 480
    • Phenotypic Enhancement: 21
    • Phenotypic Suppression: 14
    • Positive Genetic: 61
    • Synthetic Growth Defect: 49
    • Synthetic Lethality: 78
    • Synthetic Rescue: 8
    Resources
    Expression Summary
    histogram
    Resources
    Protein Information All SWI4 Protein evidence and references
    Localization
    Phosphorylation PhosphoGRID | PhosphoPep Database
    Structure
    Homologs
    sequence information
    ChrV:385876 to 382595 | ORF Map | GBrowse
    Note: this feature is encoded on the Crick strand.
    SGD ORF map
    Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
    Subfeature details
    Relative
    Coordinates    		 Chromosomal
    Coordinates    		 Most Recent Updates
    Coordinates Sequence
    CDS 1..3282 385876..382595 2011-02-03 1996-07-31
    Retrieve sequences
    Analyze Sequence
    S288C only
    S288C vs. other species
    S288C vs. other strains
    Resources
    External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
    Primary SGDIDS000000913
    References cited on this page View Complete Literature Guide for SWI4
    1) Stern M, et al.  (1984) Five SWI genes are required for expression of the HO gene in yeast. J Mol Biol 178(4):853-68
    2) Andrews BJ and Moore LA  (1992) Interaction of the yeast Swi4 and Swi6 cell cycle regulatory proteins in vitro. Proc Natl Acad Sci U S A 89(24):11852-6
    3) Andrews BJ and Herskowitz I  (1989) The yeast SWI4 protein contains a motif present in developmental regulators and is part of a complex involved in cell-cycle-dependent transcription. Nature 342(6251):830-3
    4) Ogas J, et al.  (1991) Transcriptional activation of CLN1, CLN2, and a putative new G1 cyclin (HCS26) by SWI4, a positive regulator of G1-specific transcription. Cell 66(5):1015-26
    5) Nasmyth K and Dirick L  (1991) The role of SWI4 and SWI6 in the activity of G1 cyclins in yeast. Cell 66(5):995-1013
    6) Sidorova J and Breeden L  (1993) Analysis of the SWI4/SWI6 protein complex, which directs G1/S-specific transcription in Saccharomyces cerevisiae. Mol Cell Biol 13(2):1069-77
    7) Spellman PT, et al.  (1998) Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization. Mol Biol Cell 9(12):3273-97
    8) Harrington LA and Andrews BJ  (1996) Binding to the yeast SwI4,6-dependent cell cycle box, CACGAAA, is cell cycle regulated in vivo. Nucleic Acids Res 24(4):558-65
    9) Corcoles-Saez I, et al.  (2012) Low temperature highlights the functional role of the cell wall integrity pathway in the regulation of growth in Saccharomyces cerevisiae. Biochem J 446(3):477-88
    10) Kaluarachchi Duffy S, et al.  (2012) Exploring the yeast acetylome using functional genomics. Cell 149(4):936-48
    11) Taba MR, et al.  (1991) Changes in a SWI4,6-DNA-binding complex occur at the time of HO gene activation in yeast. Genes Dev 5(11):2000-13
    12) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104
    13) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87