SWI4/YER111C Summary Help

Standard Name SWI4 1
Systematic Name YER111C
Alias ART1
Feature Type ORF, Verified
Description DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; Slt2p-independent regulator of cold growth; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p (2, 3, 4, 5, 6, 7, 8, 9, 10)
Name Description SWItching deficient 1
Chromosomal Location
ChrV:385876 to 382595 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All SWI4 GO evidence and references
  View Computational GO annotations for SWI4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 224 genes
Regulators 8 genes
Classical genetics
Large-scale survey
813 total interaction(s) for 420 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 16
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 27
  • Biochemical Activity: 2
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 2
  • Reconstituted Complex: 8
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 32
  • Negative Genetic: 485
  • Phenotypic Enhancement: 23
  • Phenotypic Suppression: 14
  • Positive Genetic: 60
  • Synthetic Growth Defect: 50
  • Synthetic Lethality: 78
  • Synthetic Rescue: 8

Expression Summary
Length (a.a.) 1,093
Molecular Weight (Da) 123,805
Isoelectric Point (pI) 9.42
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrV:385876 to 382595 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3282 385876..382595 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000913
References cited on this page View Complete Literature Guide for SWI4
1) Stern M, et al.  (1984) Five SWI genes are required for expression of the HO gene in yeast. J Mol Biol 178(4):853-68
2) Andrews BJ and Moore LA  (1992) Interaction of the yeast Swi4 and Swi6 cell cycle regulatory proteins in vitro. Proc Natl Acad Sci U S A 89(24):11852-6
3) Andrews BJ and Herskowitz I  (1989) The yeast SWI4 protein contains a motif present in developmental regulators and is part of a complex involved in cell-cycle-dependent transcription. Nature 342(6251):830-3
4) Ogas J, et al.  (1991) Transcriptional activation of CLN1, CLN2, and a putative new G1 cyclin (HCS26) by SWI4, a positive regulator of G1-specific transcription. Cell 66(5):1015-26
5) Nasmyth K and Dirick L  (1991) The role of SWI4 and SWI6 in the activity of G1 cyclins in yeast. Cell 66(5):995-1013
6) Sidorova J and Breeden L  (1993) Analysis of the SWI4/SWI6 protein complex, which directs G1/S-specific transcription in Saccharomyces cerevisiae. Mol Cell Biol 13(2):1069-77
7) Spellman PT, et al.  (1998) Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization. Mol Biol Cell 9(12):3273-97
8) Harrington LA and Andrews BJ  (1996) Binding to the yeast SwI4,6-dependent cell cycle box, CACGAAA, is cell cycle regulated in vivo. Nucleic Acids Res 24(4):558-65
9) Corcoles-Saez I, et al.  (2012) Low temperature highlights the functional role of the cell wall integrity pathway in the regulation of growth in Saccharomyces cerevisiae. Biochem J 446(3):477-88
10) Kaluarachchi Duffy S, et al.  (2012) Exploring the yeast acetylome using functional genomics. Cell 149(4):936-48
11) Taba MR, et al.  (1991) Changes in a SWI4,6-DNA-binding complex occur at the time of HO gene activation in yeast. Genes Dev 5(11):2000-13
12) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104
13) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87