GCD11/YER025W Summary Help

Standard Name GCD11 1
Systematic Name YER025W
Alias SUI4 2
Feature Type ORF, Verified
Description Gamma subunit of the translation initiation factor eIF2; involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met; mutations in human ortholog cause X-linked intellectual disability (XLID) (3, 4)
Name Description General Control Derepressed 5
Chromosomal Location
ChrV:205251 to 206834 | ORF Map | GBrowse
Gbrowse
Genetic position: 14 cM
Gene Ontology Annotations All GCD11 GO evidence and references
  View Computational GO annotations for GCD11
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 10 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
reduction of function
Resources
235 total interaction(s) for 141 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 114
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 22
  • Co-fractionation: 2
  • PCA: 1
  • Reconstituted Complex: 12
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 8
  • Phenotypic Suppression: 4
  • Positive Genetic: 52
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 3
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 527
Molecular Weight (Da) 57,865
Isoelectric Point (pI) 6.77
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:205251 to 206834 | ORF Map | GBrowse
SGD ORF map
Genetic position: 14 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1584 205251..206834 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000827
References cited on this page View Complete Literature Guide for GCD11
1) Harashima S and Hinnebusch AG  (1986) Multiple GCD genes required for repression of GCN4, a transcriptional activator of amino acid biosynthetic genes in Saccharomyces cerevisiae. Mol Cell Biol 6(11):3990-8
2) Huang HK, et al.  (1997) GTP hydrolysis controls stringent selection of the AUG start codon during translation initiation in Saccharomyces cerevisiae. Genes Dev 11(18):2396-413
3) Laurino JP, et al.  (1999) The beta subunit of eukaryotic translation initiation factor 2 binds mRNA through the lysine repeats and a region comprising the C2-C2 motif. Mol Cell Biol 19(1):173-81
4) Borck G, et al.  (2012) eIF2? mutation that disrupts eIF2 complex integrity links intellectual disability to impaired translation initiation. Mol Cell 48(4):641-6
5) Lucchini G, et al.  (1984) Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae. Mol Cell Biol 4(7):1326-33