SRB4/YER022W Summary

SRB4 BASIC INFORMATION

Standard Name	SRB4 1
Systematic Name	YER022W
Alias	MED17 2
Feature Type	ORF, Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation (1, 3)
Name Description	Suppressor of RNA polymerase B 1

GO Annotations	All SRB4 GO evidence and references
	View Computational GO annotations for SRB4
Molecular Function
Manually curated	RNA polymerase II transcription mediator activity (IDA)
Biological Process
Manually curated	transcription from RNA polymerase II promoter (IDA)
Cellular Component
Manually curated	mediator complex (IDA)

Mutant Phenotype	All SRB4 Phenotype details and references
Classical genetics
conditional	poly(A)+ mRNA accumulation: decreased heat sensitivity: increased
Large-scale survey
null	inviable

Interactions	SRB4 All interactions details and references
	248 total interaction(s) for 70 unique genes/features.
Physical Interactions	Affinity Capture-MS: 108 Affinity Capture-RNA: 1 Affinity Capture-Western: 55 Co-localization: 1 Co-purification: 50 Reconstituted Complex: 2 Two-hybrid: 14
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 1 Phenotypic Enhancement: 4 Synthetic Rescue: 11

Sequence Information

ChrV:198811 to 200874 | |

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2064	198811..200874	1996-07-31	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000000824

SRB4 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for SRB4

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SRB4]

1)	Thompson CM, et al. (1993) A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast. Cell 73(7):1361-75
2)	Bourbon HM, et al. (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
3)	Kornberg RD (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9

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