YEN1/YER041W Summary Help

Standard Name YEN1 1
Systematic Name YER041W
Feature Type ORF, Verified
Description Holliday junction resolvase; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1; similar to S. cerevisiae endonuclease Rth1p (1, 2, 3, 4, 5)
Chromosomal Location
ChrV:232461 to 234740 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All YEN1 GO evidence and references
  View Computational GO annotations for YEN1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 7 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
44 total interaction(s) for 21 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 2
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Phenotypic Enhancement: 9
  • Phenotypic Suppression: 7
  • Synthetic Growth Defect: 14
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 759
Molecular Weight (Da) 87,364
Isoelectric Point (pI) 7.26
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:232461 to 234740 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2280 232461..234740 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000843
References cited on this page View Complete Literature Guide for YEN1
1) Johnson RE, et al.  (1998) Role of yeast Rth1 nuclease and its homologs in mutation avoidance, DNA repair, and DNA replication. Curr Genet 34(1):21-9
2) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
3) Ip SC, et al.  (2008) Identification of Holliday junction resolvases from humans and yeast. Nature 456(7220):357-361
4) Kosugi S, et al.  (2009) Systematic identification of cell cycle-dependent yeast nucleocytoplasmic shuttling proteins by prediction of composite motifs. Proc Natl Acad Sci U S A 106(25):10171-6
5) Pardo B and Aguilera A  (2012) Complex chromosomal rearrangements mediated by break-induced replication involve structure-selective endonucleases. PLoS Genet 8(9):e1002979