NPR2/YEL062W Summary Help

Standard Name NPR2 1
Systematic Name YEL062W
Feature Type ORF, Verified
Description Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human tumor suppressor NPRL2; target of Grr1p; required for growth on urea and proline (1, 2, 3, 4, 5, 6)
Name Description Nitrogen Permease Regulator 1
Chromosomal Location
ChrV:34407 to 36254 | ORF Map | GBrowse
Gene Ontology Annotations All NPR2 GO evidence and references
  View Computational GO annotations for NPR2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Classical genetics
Large-scale survey
39 total interaction(s) for 25 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 18
  • Affinity Capture-Western: 6
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 4
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 6

Expression Summary
Length (a.a.) 615
Molecular Weight (Da) 69,858
Isoelectric Point (pI) 8.62
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrV:34407 to 36254 | ORF Map | GBrowse
Last Update Coordinates: 2003-09-22 | Sequence: 2003-09-22
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1848 34407..36254 2003-09-22 2003-09-22
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000788
References cited on this page View Complete Literature Guide for NPR2
1) Rousselet G, et al.  (1995) A second nitrogen permease regulator in Saccharomyces cerevisiae. FEBS Lett 359(2-3):215-9
2) Schenk PW, et al.  (2003) Anticancer drug resistance induced by disruption of the Saccharomyces cerevisiae NPR2 gene: a novel component involved in cisplatin- and doxorubicin-provoked cell kill. Mol Pharmacol 64(2):259-68
3) Neklesa TK and Davis RW  (2009) A Genome-Wide Screen for Regulators of TORC1 in Response to Amino Acid Starvation Reveals a Conserved Npr2/3 Complex. PLoS Genet 5(6):e1000515
4) Spielewoy N, et al.  (2010) Npr2, yeast homolog of the human tumor suppressor NPRL2, is a target of Grr1 required for adaptation to growth on diverse nitrogen sources. Eukaryot Cell 9(4):592-601
5) Dokudovskaya S, et al.  (2011) A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae. Mol Cell Proteomics 10(6):M110.006478
6) Wu X and Tu BP  (2011) Selective regulation of autophagy by the Iml1-Npr2-Npr3 complex in the absence of nitrogen starvation. Mol Biol Cell 22(21):4124-33