MMS21/YEL019C Summary Help

Standard Name MMS21 1
Systematic Name YEL019C
Alias NSE2 2 , PSO10 3
Feature Type ORF, Verified
Description SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination; SUMOylates and inhibits Snf1p function (1, 4, 5, 6, 7)
Name Description Methyl MethaneSulfonate sensitivity 1
Chromosomal Location
ChrV:121301 to 120498 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All MMS21 GO evidence and references
  View Computational GO annotations for MMS21
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
reduction of function
Large-scale survey
86 total interaction(s) for 43 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-Western: 2
  • Biochemical Activity: 3
  • Co-crystal Structure: 1
  • Co-fractionation: 1
  • Co-purification: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 4
  • Negative Genetic: 1
  • Phenotypic Enhancement: 7
  • Phenotypic Suppression: 3
  • Synthetic Growth Defect: 22
  • Synthetic Lethality: 20
  • Synthetic Rescue: 8

Expression Summary
Length (a.a.) 267
Molecular Weight (Da) 30,354
Isoelectric Point (pI) 4.9
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrV:121301 to 120498 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..804 121301..120498 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000745
References cited on this page View Complete Literature Guide for MMS21
1) Prakash S and Prakash L  (1977) Increased spontaneous mitotic segregation in MMS-sensitive mutants of Saccharomyces cerevisiae. Genetics 87(2):229-36
2) Murray JM and Carr AM  (2008) Smc5/6: a link between DNA repair and unidirectional replication? Nat Rev Mol Cell Biol 9(2):177-82
3) Hoch NC, et al.  (2008) Allelism of Saccharomyces cerevisiae gene PSO10, involved in error-prone repair of psoralen-induced DNA damage, with SUMO ligase-encoding MMS21. Curr Genet 53(6):361-71
4) Montelone BA and Koelliker KJ  (1995) Interactions among mutations affecting spontaneous mutation, mitotic recombination, and DNA repair in yeast. Curr Genet 27(2):102-9
5) Zhao X and Blobel G  (2005) A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization. Proc Natl Acad Sci U S A 102(13):4777-82
6) Bermudez-Lopez M, et al.  (2010) The Smc5/6 complex is required for dissolution of DNA-mediated sister chromatid linkages. Nucleic Acids Res 38(19):6502-12
7) Simpson-Lavy KJ and Johnston M  (2013) SUMOylation regulates the SNF1 protein kinase. Proc Natl Acad Sci U S A 110(43):17432-7