Standard Name	UBC8 1
Systematic Name	YEL012W
Alias	GID3 2 , 3
Feature Type	ORF, Verified
Description	Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro (1, 4, 5)
Name Description	UBiquitin-Conjugating 1

Chromosomal Location	ChrV:131772 to 132551 | ORF Map | GBrowse

	View Computational GO annotations for UBC8
Molecular Function
Manually curated	ubiquitin-protein ligase activity (IDA, ISS)
Biological Process
Manually curated	negative regulation of gluconeogenesis (IMP) proteasomal ubiquitin-dependent protein catabolic process (IMP) protein monoubiquitination (TAS) protein polyubiquitination (TAS) protein ubiquitination involved in ubiquitin-dependent protein catabolic process (IMP)
Cellular Component
Manually curated	cytoplasm (IDA)

Classical genetics
null	Myc9-Gid4p accumulation: increased Fbp1p accumulation: increased resistance to sirolimus: decreased
Large-scale survey
null	glutathione excretion: increased chronological lifespan: decreased competitive fitness: decreased haploinsufficient heat sensitivity: increased resistance to quinine: decreased resistance to arsenite(3-): decreased resistance to tunicamycin: increased resistance to fluconazole: increased resistance to fenpropimorph: increased resistance to cycloheximide: increased resistance to camptothecin: increased resistance to bleomycin: decreased resistance to caffeine: decreased resistance to sirolimus: decreased resistance to methyl methanesulfonate: decreased resistance to benzo[a]pyrene: decreased respiratory growth: decreased sporulation efficiency: decreased toxin resistance: decreased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	85 total interaction(s) for 83 unique genes/features.
Physical Interactions	Affinity Capture-MS: 3 Affinity Capture-RNA: 1 Affinity Capture-Western: 2 Biochemical Activity: 1 Reconstituted Complex: 6 Two-hybrid: 1
Genetic Interactions	Negative Genetic: 65 Positive Genetic: 5 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrV:131772 to 132551 | ORF Map | GBrowse
Last Update	Coordinates: 2000-12-01 | Sequence: 2000-12-01
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..5 131772..131776 2000-12-01 2000-12-01 Intron 6..128 131777..131899 2000-12-01 2000-12-01 CDS 129..780 131900..132551 2000-12-01 2000-12-01
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS) Intron (6 - 128)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000738