VPS3/YDR495C Summary Help

Standard Name VPS3 1, 2
Systematic Name YDR495C
Alias PEP6 , VPL3 3 , VPT17 1
Feature Type ORF, Verified
Description Component of CORVET membrane tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase (1, 4, 5, 6, 7)
Name Description Vacuolar Protein Sorting 1, 2
Chromosomal Location
ChrIV:1441150 to 1438115 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All VPS3 GO evidence and references
  View Computational GO annotations for VPS3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
overexpression
reduction of function
Large-scale survey
null
Resources
132 total interaction(s) for 84 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 13
  • Reconstituted Complex: 4
  • Two-hybrid: 11

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Rescue: 2
  • Negative Genetic: 15
  • Phenotypic Enhancement: 29
  • Phenotypic Suppression: 19
  • Positive Genetic: 2
  • Synthetic Growth Defect: 16
  • Synthetic Lethality: 9

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,011
Molecular Weight (Da) 116,921
Isoelectric Point (pI) 4.87
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1441150 to 1438115 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3036 1441150..1438115 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002903
References cited on this page View Complete Literature Guide for VPS3
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Rothman JH and Stevens TH  (1986) Protein sorting in yeast: mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway. Cell 47(6):1041-51
4) Rothman JH, et al.  (1989) Acidification of the lysosome-like vacuole and the vacuolar H+-ATPase are deficient in two yeast mutants that fail to sort vacuolar proteins. J Cell Biol 109(1):93-100
5) Raymond CK, et al.  (1990) Molecular analysis of the yeast VPS3 gene and the role of its product in vacuolar protein sorting and vacuolar segregation during the cell cycle. J Cell Biol 111(3):877-92
6) Peplowska K, et al.  (2007) The CORVET Tethering Complex Interacts with the Yeast Rab5 Homolog Vps21 and Is Involved in Endo-Lysosomal Biogenesis. Dev Cell 12(5):739-50
7) Balderhaar HJ, et al.  (2013) The CORVET complex promotes tethering and fusion of Rab5/Vps21-positive membranes. Proc Natl Acad Sci U S A 110(10):3823-8