NHX1/YDR456W Summary Help

Standard Name NHX1 1
Systematic Name YDR456W
Alias VPS44 2 , NHA2 3 , VPL27 2
Feature Type ORF, Verified
Description Na+/H+ and K+/H+ exchanger; required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion; ortholog of human NHE9, which is linked to autism (1, 4, 5, 6, 7)
Name Description Na+/H+ eXchanger 1
Chromosomal Location
ChrIV:1367485 to 1369386 | ORF Map | GBrowse
Gene Ontology Annotations All NHX1 GO evidence and references
  View Computational GO annotations for NHX1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
reduction of function
Large-scale survey
209 total interaction(s) for 164 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 1
  • PCA: 18
  • Reconstituted Complex: 1
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 102
  • Phenotypic Enhancement: 24
  • Phenotypic Suppression: 13
  • Positive Genetic: 4
  • Synthetic Growth Defect: 31
  • Synthetic Lethality: 6
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 633
Molecular Weight (Da) 70,147
Isoelectric Point (pI) 4.5
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:1367485 to 1369386 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1902 1367485..1369386 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000002864
References cited on this page View Complete Literature Guide for NHX1
1) Nass R, et al.  (1997) Intracellular sequestration of sodium by a novel Na+/H+ exchanger in yeast is enhanced by mutations in the plasma membrane H+-ATPase. Insights into mechanisms of sodium tolerance. J Biol Chem 272(42):26145-52
2) Raymond CK, et al.  (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402
3) Numata M, et al.  (1998) Identification of a mitochondrial Na+/H+ exchanger. J Biol Chem 273(12):6951-9
4) Nass R and Rao R  (1999) The yeast endosomal Na+/H+ exchanger, Nhx1, confers osmotolerance following acute hypertonic shock. Microbiology 145 ( Pt 11)():3221-8
5) Brett CL, et al.  (2005) The yeast endosomal Na+K+/H+ exchanger Nhx1 regulates cellular pH to control vesicle trafficking. Mol Biol Cell 16(3):1396-405
6) Qiu QS and Fratti RA  (2010) The Na+/H+ exchanger Nhx1p regulates the initiation of Saccharomyces cerevisiae vacuole fusion. J Cell Sci 123(Pt 19):3266-75
7) Kondapalli KC, et al.  (2013) Functional evaluation of autism-associated mutations in NHE9. Nat Commun 4():2510