NHX1/YDR456W Summary

NHX1/YDR456W Summary

Standard Name	NHX1 1
Systematic Name	YDR456W
Alias	VPS44 2 , NHA2 3 , VPL27 2
Feature Type	ORF, Verified
Description	Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion (1, 4, 5, 6)
Name Description	Na+/H+ eXchanger 1

Chromosomal Location	ChrIV:1367485 to 1369386 \| ORF Map \| GBrowse

Gene Ontology Annotations All NHX1 GO evidence and references

	View Computational GO annotations for NHX1
Molecular Function
Manually curated	potassium:hydrogen antiporter activity (IMP) sodium:hydrogen antiporter activity (IMP)
Biological Process
Manually curated	cellular monovalent inorganic cation homeostasis (IMP) potassium ion transmembrane transport (IMP) sodium ion transmembrane transport (IMP) vacuolar acidification (IMP)
Cellular Component
Manually curated	early endosome (IDA) fungal-type vacuole membrane (IDA) late endosome (IDA) trans-Golgi network (IDA)
High-throughput	integral to membrane (ISM)

Mutant phenotypes All NHX1 Phenotype evidence and references

Classical genetics
null	acid pH resistance: decreased chloride accumulation: decreased hyperosmotic stress resistance: decreased ionic stress resistance: decreased resistance to hygromycin B: decreased resistance to gentamycin: decreased vacuolar morphology: abnormal viable
Large-scale survey
null	rubidium(1+) accumulation: decreased glutathione excretion: increased haploinsufficient hyperosmotic stress resistance: decreased metal resistance: decreased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to (S)-lactic acid: decreased resistance to hygromycin B: decreased resistance to spermine: decreased resistance to chloroquine: decreased resistance to ciclopirox olamine: decreased resistance to BPS: decreased resistance to benzo[a]pyrene: decreased respiratory growth: decreased rate stress resistance: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
overexpression	vegetative growth: decreased rate vegetative growth: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All NHX1 Interaction evidence and references

	115 total interaction(s) for 85 unique genes/features.
Physical Interactions	Affinity Capture-RNA: 2 Affinity Capture-Western: 1 PCA: 18 Reconstituted Complex: 1 Two-hybrid: 2
Genetic Interactions	Dosage Rescue: 2 Negative Genetic: 15 Phenotypic Enhancement: 24 Phenotypic Suppression: 13 Positive Genetic: 1 Synthetic Growth Defect: 29 Synthetic Lethality: 6 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All NHX1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:1367485 to 1369386 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1902	1367485..1369386	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| TCDB \| UniProtKB

Primary SGDID	S000002864

References cited on this page View Complete Literature Guide for NHX1

1)	Nass R, et al. (1997) Intracellular sequestration of sodium by a novel Na+/H+ exchanger in yeast is enhanced by mutations in the plasma membrane H+-ATPase. Insights into mechanisms of sodium tolerance. J Biol Chem 272(42):26145-52
2)	Raymond CK, et al. (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402
3)	Numata M, et al. (1998) Identification of a mitochondrial Na+/H+ exchanger. J Biol Chem 273(12):6951-9
4)	Nass R and Rao R (1999) The yeast endosomal Na+/H+ exchanger, Nhx1, confers osmotolerance following acute hypertonic shock. Microbiology 145 ( Pt 11):3221-8
5)	Brett CL, et al. (2005) The yeast endosomal Na+K+/H+ exchanger Nhx1 regulates cellular pH to control vesicle trafficking. Mol Biol Cell 16(3):1396-405
6)	Qiu QS and Fratti RA (2010) The Na+/H+ exchanger Nhx1p regulates the initiation of Saccharomyces cerevisiae vacuole fusion. J Cell Sci 123(Pt 19):3266-75