SSN2/YDR443C Summary

SSN2 BASIC INFORMATION

Standard Name	SSN2 1
Systematic Name	YDR443C
Alias	NUT8 , SCA1 , SRB9 , UME2 , RYE3 , MED13 2
Feature Type	ORF, Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation (1, 3, 4)
Name Description	Suppressor of SNf1 1

GO Annotations	All SSN2 GO evidence and references
	View Computational GO annotations for SSN2
Molecular Function
Manually curated	general RNA polymerase II transcription factor activity (TAS)
Biological Process
Manually curated	negative regulation of transcription from RNA polymerase II promoter (TAS) nuclear-transcribed mRNA catabolic process, non-stop decay (IMP)
Cellular Component
Manually curated	mediator complex (IDA) transcription factor complex (TAS)

Mutant Phenotype	All SSN2 Phenotype details and references
Classical genetics
unspecified	flocculation: increased heat sensitivity: increased survival rate in stationary phase: decreased
Large-scale survey
null	Protein A-nonstop reporter mRNA accumulation: increased competitive fitness: decreased resistance to selenomethionine: increased resistance to 5-fluorouracil: decreased resistance to nickel sulfate: increased sporulation: decreased telomere length: increased viable
overexpression	vegetative growth: decreased

Interactions	SSN2 All interactions details and references
	195 total interaction(s) for 149 unique genes/features.
Physical Interactions	Affinity Capture-MS: 47 Affinity Capture-RNA: 1 Affinity Capture-Western: 7 Biochemical Activity: 2 Two-hybrid: 9
Genetic Interactions	Dosage Rescue: 5 Phenotypic Enhancement: 58 Phenotypic Suppression: 37 Synthetic Growth Defect: 17 Synthetic Lethality: 6 Synthetic Rescue: 6

Sequence Information

ChrIV:1349931 to 1345669 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2008-06-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..4263	1349931..1345669	2008-06-05	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000002851

SSN2 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for SSN2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SSN2]

1)	Carlson M, et al. (1984) A suppressor of SNF1 mutations causes constitutive high-level invertase synthesis in yeast. Genetics 107(1):19-32
2)	Bourbon HM, et al. (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
3)	Song W, et al. (1996) SSN genes that affect transcriptional repression in Saccharomyces cerevisiae encode SIN4, ROX3, and SRB proteins associated with RNA polymerase II. Mol Cell Biol 16(1):115-20
4)	Larschan E and Winston F (2005) The Saccharomyces cerevisiae Srb8-Srb11 complex functions with the SAGA complex during Gal4-activated transcription. Mol Cell Biol 25(1):114-23

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