DOT1/YDR440W Summary

DOT1/YDR440W Summary

Standard Name	DOT1 1
Systematic Name	YDR440W
Alias	PCH1 2
Feature Type	ORF, Verified
Description	Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response (2, 3, 4, 5)
Name Description	Disruptor Of Telomeric silencing 1
Gene Product Alias	KMT4 6

Chromosomal Location	ChrIV:1342493 to 1344241 \| ORF Map \| GBrowse

Gene Ontology Annotations All DOT1 GO evidence and references

	View Computational GO annotations for DOT1
Molecular Function
Manually curated	histone methyltransferase activity (H3-K79 specific) (IDA, IMP) nucleosomal histone binding (IDA)
Biological Process
Manually curated	chromatin silencing at telomere (IMP) DNA damage checkpoint (IGI, IMP) G1 DNA damage checkpoint (IMP) global genome nucleotide-excision repair (IMP) histone H3-K79 methylation (IDA, IMP) intra-S DNA damage checkpoint (IMP) meiotic recombination checkpoint (IGI) nucleotide-excision repair (IGI, IMP) postreplication repair (IGI) recombinational repair (IGI, IMP)
Cellular Component
Manually curated	nucleus (IDA)

Mutant phenotypes All DOT1 Phenotype evidence and references

Classical genetics
null	X ray resistance: increased tellurium atom accumulation: increased gamma ray resistance: decreased mutation frequency: increased Rad52-YFP distribution: increased histone H2A modification: increased histone H3 modification: increased resistance to tellurite: decreased resistance to methyl methanesulfonate: increased silencing: decreased telomere length: abnormal
overexpression	UTP22 mRNA accumulation: increased silencing: decreased
Large-scale survey
null	chitin deposition: decreased haploinsufficient resistance to tunicamycin: decreased resistance to benzo[a]pyrene: decreased resistance to cycloheximide: increased resistance to caffeine: increased resistance to methyl methanesulfonate: increased resistance to 2,4-Diacetylphloroglucinol: decreased resistance to fenpropimorph: decreased resistance to Calcofluor White: normal vacuolar morphology: abnormal viable
overexpression	silencing: decreased silencing: abnormal vegetative growth: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All DOT1 Interaction evidence and references

	249 total interaction(s) for 168 unique genes/features.
Physical Interactions	Affinity Capture-MS: 6 Affinity Capture-RNA: 1 Affinity Capture-Western: 6 Biochemical Activity: 14 Co-localization: 3 Far Western: 1 Two-hybrid: 1
Genetic Interactions	Dosage Growth Defect: 5 Dosage Rescue: 2 Negative Genetic: 125 Phenotypic Enhancement: 18 Phenotypic Suppression: 6 Positive Genetic: 3 Synthetic Growth Defect: 34 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 8 Synthetic Rescue: 15
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All DOT1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:1342493 to 1344241 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1749	1342493..1344241	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000002848

References cited on this page View Complete Literature Guide for DOT1

1)	Singer MS, et al. (1998) Identification of high-copy disruptors of telomeric silencing in Saccharomyces cerevisiae. Genetics 150(2):613-32
2)	San-Segundo PA and Roeder GS (2000) Role for the silencing protein Dot1 in meiotic checkpoint control. Mol Biol Cell 11(10):3601-15
3)	van Leeuwen F, et al. (2002) Dot1p modulates silencing in yeast by methylation of the nucleosome core. Cell 109(6):745-56
4)	Game JC, et al. (2005) X-ray survival characteristics and genetic analysis for nine Saccharomyces deletion mutants that show altered radiation sensitivity. Genetics 169(1):51-63
5)	Wysocki R, et al. (2005) Role of Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9. Mol Cell Biol 25(19):8430-43
6)	Allis CD, et al. (2007) New nomenclature for chromatin-modifying enzymes. Cell 131(4):633-6