CAD1/YDR423C Summary

Standard Name	CAD1 1
Systematic Name	YDR423C
Alias	YAP2
Feature Type	ORF, Verified
Description	AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7, 8, 9)
Name Description	CADmium resistance 10

Chromosomal Location	ChrIV:1319275 to 1318046 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

	Genetic position: 271 cM

Gene Ontology Annotations All CAD1 GO evidence and references

	View Computational GO annotations for CAD1
Molecular Function
Manually curated	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription (IDA) sequence-specific DNA binding (IDA)
Biological Process
Manually curated	cellular response to cadmium ion (IDA, IGI, IMP) positive regulation of transcription from RNA polymerase II promoter (IDA) positive regulation of transcription from RNA polymerase II promoter in response to stress (IGI)
Cellular Component
Manually curated	cytoplasm (IDA) nucleus (IC, IDA)

Regulatory Role

Binding motifs	Predicted CAD1 Binding Site Locations
	MTTASTMAKC 11 [Search intergenic regions for MTTASTMAKC] TTACTAA 6 [Search intergenic regions for TTACTAA]

Regulatory modules	predicted: stressResponse (649, 439, 427, 367)

Resources	ScerTF \| YEASTRACT \| YeTFaSCo

Mutant phenotypes All CAD1 Phenotype evidence and references

Classical genetics
null	metal resistance: normal oxidative stress resistance: decreased viable
overexpression	metal resistance: increased metal resistance: normal resistance to 1,10-phenanthroline: increased resistance to cycloheximide: increased resistance to cadmium(2+): increased
Large-scale survey
null	competitive fitness: increased haploinsufficient resistance to 5-fluorouracil: decreased resistance to neomycin: decreased resistance to cycloheximide: decreased viable
overexpression	filamentous growth: increased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All CAD1 Interaction evidence and references

	36 total interaction(s) for 32 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-RNA: 2 Affinity Capture-Western: 2 Two-hybrid: 6
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 14 Phenotypic Enhancement: 2 Positive Genetic: 2 Synthetic Growth Defect: 5 Synthetic Lethality: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All CAD1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:1319275 to 1318046 | |

Note: this feature is encoded on the Crick strand.

: 271 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1230	1319275..1318046	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000002831

References cited on this page View Complete Literature Guide for CAD1

1)	Januska, A. (1989) Personal Communication, Mortimer Map Edition 10
2)	Lesuisse E and Labbe P (1995) Effects of cadmium and of YAP1 and CAD1/YAP2 genes on iron metabolism in the yeast Saccharomyces cerevisiae. Microbiology 141 ( Pt 11):2937-43
3)	Vilela C, et al. (1998) The yeast transcription factor genes YAP1 and YAP2 are subject to differential control at the levels of both translation and mRNA stability. Nucleic Acids Res 26(5):1150-9
4)	Hirata D, et al. (1994) Stress-induced transcriptional activation mediated by YAP1 and YAP2 genes that encode the Jun family of transcriptional activators in Saccharomyces cerevisiae. Mol Gen Genet 242(3):250-6
5)	Miyahara K, et al. (1996) yAP-1- and yAP-2-mediated, heat shock-induced transcriptional activation of the multidrug resistance ABC transporter genes in Saccharomyces cerevisiae. Curr Genet 29(2):103-5
6)	Fernandes L, et al. (1997) Yap, a novel family of eight bZIP proteins in Saccharomyces cerevisiae with distinct biological functions. Mol Cell Biol 17(12):6982-93
7)	Vilela C, et al. (1999) Post-termination ribosome interactions with the 5'UTR modulate yeast mRNA stability. EMBO J 18(11):3139-52
8)	Cohen BA, et al. (2002) Discrimination between paralogs using microarray analysis: application to the Yap1p and Yap2p transcriptional networks. Mol Biol Cell 13(5):1608-14
9)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
10)	Wu A, et al. (1993) Yeast bZip proteins mediate pleiotropic drug and metal resistance. J Biol Chem 268(25):18850-8
11)	Harbison CT, et al. (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104