RAD30/YDR419W Summary Help

Standard Name RAD30 1
Systematic Name YDR419W
Alias DBH1
Feature Type ORF, Verified
Description DNA polymerase eta; involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; involved in formation of post-replicative damage-induced genome-wide cohesion; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV (2, 3, 4, 5, 6, 7 and see Summary Paragraph)
Name Description RADiation sensitive 1
Chromosomal Location
ChrIV:1303174 to 1305072 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RAD30 GO evidence and references
  View Computational GO annotations for RAD30
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
Resources
145 total interaction(s) for 83 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 2
  • PCA: 4
  • Reconstituted Complex: 3
  • Two-hybrid: 8

Genetic Interactions
  • Dosage Lethality: 2
  • Negative Genetic: 32
  • Phenotypic Enhancement: 45
  • Phenotypic Suppression: 6
  • Positive Genetic: 5
  • Synthetic Growth Defect: 17
  • Synthetic Lethality: 7
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 632
Molecular Weight (Da) 71,514
Isoelectric Point (pI) 8.22
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1303174 to 1305072 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1899 1303174..1305072 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002827
SUMMARY PARAGRAPH for RAD30

RAD30 encodes DNA polymerase eta, a member of the Y-family of DNA polymerases involved in translesion synthesis during post-replication repair (8, 9). Rad30p incorporates nucleotides across a wide range of DNA lesions, such as UV-induced dimers, damaged base pairs, and abasic sites that can cause replication forks to stall (reviewed in 5). Translesion synthesis can be error-free or error-prone depending on the damaged nucleotide and the nucleotide inserted by Rad30p (2, 3, 10, 11, 12, 13, 14). However, Rad30p is commonly considered to be involved in error-free translesion synthesis because it can correctly insert two A's across the most abundant UV-induced lesion, the cis-syn thymine-thymine dimer (also known as cyclobutane pyrimidine dimer or CPD) (15, 5).

Rad30p is recruited to stalled replication forks following the monoubiquitination of PCNA by Rad6p-Rad18p in response to DNA damage (8, 9). Pol eta can replace pol delta in the replication holoenzyme (16). Rad30p has a large active site which allows it to tolerate bulky adducts such CPD (17). Like other members of the Y-family of DNA polymerases, Rad30p has low processivity, low fidelity, and no proofreading exonuclease activity (18). Its processivity can be influenced by the type of DNA lesion. For example, Rad30p alone can continue synthesis past an 8oxoG lesion but cooperates with pol zeta to extend replication after inserting nucleotides across a (6-4) thymine-thymine lesion (3, 10, 19, 12).

Rad30p contains a UBZ (ubiquitin binding zinc finger) and PIP (PCNA interacting peptide) motif (5). The interaction with PCNA is essential for Rad30 activity (20, 16). Rad30p is regulated by UV irradiation (21, 22).

Although the Y-family of DNA polymerases are widely conserved, the subfamily of polymerases related to Rad30p are only found in eukaryotes (5, 23). In humans, mutations in pol eta are responsible for xeroderma pigmentosum, variant type (XPV) (24, 4).

Last updated: 2010-01-19 Contact SGD

References cited on this page View Complete Literature Guide for RAD30
1) McDonald JP, et al.  (1997) The Saccharomyces cerevisiae RAD30 gene, a homologue of Escherichia coli dinB and umuC, is DNA damage inducible and functions in a novel error-free postreplication repair mechanism. Genetics 147(4):1557-68
2) Johnson RE, et al.  (1999) Efficient bypass of a thymine-thymine dimer by yeast DNA polymerase, Poleta. Science 283(5404):1001-4
3) Yuan F, et al.  (2000) Specificity of DNA lesion bypass by the yeast DNA polymerase eta. J Biol Chem 275(11):8233-9
4) Masutani C, et al.  (1999) The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase eta. Nature 399(6737):700-4
5) Prakash S, et al.  (2005) Eukaryotic translesion synthesis DNA polymerases: specificity of structure and function. Annu Rev Biochem 74:317-53
6) Enervald E, et al.  (2013) Importance of poleta for damage-induced cohesion reveals differential regulation of cohesion establishment at the break site and genome-wide. PLoS Genet 9(1):e1003158
7) Chatterjee N, et al.  (2013) Role of polymerase eta in mitochondrial mutagenesis of Saccharomyces cerevisiae. Biochem Biophys Res Commun 431(2):270-3
8) Andersen PL, et al.  (2008) Eukaryotic DNA damage tolerance and translesion synthesis through covalent modifications of PCNA. Cell Res 18(1):162-73
9) Lee KY and Myung K  (2008) PCNA Modifications for Regulation of Post-Replication Repair Pathways. Mol Cells 26(1):5-11
10) Haracska L, et al.  (2000) Efficient and accurate replication in the presence of 7,8-dihydro-8-oxoguanine by DNA polymerase eta. Nat Genet 25(4):458-61
11) Yu SL, et al.  (2001) Requirement of DNA polymerase eta for error-free bypass of UV-induced CC and TC photoproducts. Mol Cell Biol 21(1):185-8
12) Johnson RE, et al.  (2001) Role of DNA polymerase eta in the bypass of a (6-4) TT photoproduct. Mol Cell Biol 21(10):3558-63
13) Zhao B, et al.  (2004) Role of DNA polymerase eta in the bypass of abasic sites in yeast cells. Nucleic Acids Res 32(13):3984-94
14) Abdulovic AL and Jinks-Robertson S  (2006) The in Vivo Characterization of Translesion Synthesis Across UV-Induced Lesions in Saccharomyces cerevisiae: Insights Into Pol{zeta}- and Pol{eta}-Dependent Frameshift Mutagenesis. Genetics 172(3):1487-98
15) Bresson A and Fuchs RP  (2002) Lesion bypass in yeast cells: Pol eta participates in a multi-DNA polymerase process. EMBO J 21(14):3881-7
16) Zhuang Z, et al.  (2008) Regulation of polymerase exchange between Poleta and Poldelta by monoubiquitination of PCNA and the movement of DNA polymerase holoenzyme. Proc Natl Acad Sci U S A 105(14):5361-6
17) Trincao J, et al.  (2001) Structure of the catalytic core of S. cerevisiae DNA polymerase eta: implications for translesion DNA synthesis. Mol Cell 8(2):417-26
18) Washington MT, et al.  (1999) Fidelity and processivity of Saccharomyces cerevisiae DNA polymerase eta. J Biol Chem 274(52):36835-8
19) Johnson RE, et al.  (2000) Eukaryotic polymerases iota and zeta act sequentially to bypass DNA lesions. Nature 406(6799):1015-9
20) Haracska L, et al.  (2001) Interaction with PCNA is essential for yeast DNA polymerase eta function. Mol Cell 8(2):407-15
21) Skoneczna A, et al.  (2007) Polymerase eta Is a Short-lived, Proteasomally Degraded Protein that Is Temporarily Stabilized Following UV Irradiation in Saccharomyces cerevisiae. J Mol Biol 366(4):1074-86
22) Pabla R, et al.  (2008) Regulation of Saccharomyces cerevisiae DNA polymerase eta transcript and protein. Radiat Environ Biophys 47(1):157-68
23) McDonald JP, et al.  (2001) DNA polymerase iota and related rad30-like enzymes. Philos Trans R Soc Lond B Biol Sci 356(1405):53-60
24) Johnson RE, et al.  (1999) hRAD30 mutations in the variant form of xeroderma pigmentosum. Science 285(5425):263-5