MUS81/YDR386W Summary

MUS81/YDR386W Summary

Standard Name	MUS81 1
Systematic Name	YDR386W
Alias	SLX3
Feature Type	ORF, Verified
Description	Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p (1, 2, 3, 4, 5, 6)
Name Description	MMS and UV Sensitive 1

Chromosomal Location	ChrIV:1246084 to 1247982 \| ORF Map \| GBrowse

Gene Ontology Annotations All MUS81 GO evidence and references

	View Computational GO annotations for MUS81
Molecular Function
Manually curated	crossover junction endodeoxyribonuclease activity (IDA) endonuclease activity (IDA)
Biological Process
Manually curated	DNA repair (IGI) DNA topological change (IGI) reciprocal meiotic recombination (IGI) resolution of meiotic recombination intermediates (IGI) response to DNA damage stimulus (IMP)
Cellular Component
Manually curated	Holliday junction resolvase complex (IDA) nucleus (IMP, IPI)

Mutant phenotypes All MUS81 Phenotype evidence and references

Classical genetics
null	apoptosis: decreased cell cycle progression in G1 phase: decreased duration cell cycle progression in S phase: decreased duration tellurium atom accumulation: increased chromosome/plasmid maintenance: abnormal gamma ray resistance: decreased resistance to selenium(2+): decreased resistance to hydroxyurea: decreased resistance to methyl methanesulfonate: decreased
Large-scale survey
null	UV resistance: decreased competitive fitness: decreased gamma ray resistance: decreased haploproficient heat sensitivity: increased mitotic recombination: increased Rad52-YFP distribution: increased resistance to fluconazole: decreased resistance to hydroxyurea: decreased resistance to camptothecin: decreased resistance to methyl methanesulfonate: decreased resistance to tirapazamine: decreased resistance to tunicamycin: increased resistance to bleomycin: increased resistance to mycophenolic acid: decreased resistance to sodium selenide: decreased resistance to 2-phenyl-3-nitroso-imidazo[1,2-a]pyrimidine: decreased resistance to cycloheximide: decreased resistance to cisplatin: decreased resistance to doxorubicin: decreased sporulation: normal vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All MUS81 Interaction evidence and references

	259 total interaction(s) for 132 unique genes/features.
Physical Interactions	Affinity Capture-MS: 32 Affinity Capture-Western: 4 Biochemical Activity: 1 Co-crystal Structure: 1 Co-purification: 2 Reconstituted Complex: 3 Two-hybrid: 7
Genetic Interactions	Dosage Rescue: 2 Negative Genetic: 74 Phenotypic Enhancement: 11 Phenotypic Suppression: 6 Positive Genetic: 6 Synthetic Growth Defect: 63 Synthetic Lethality: 41 Synthetic Rescue: 6
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All MUS81 Protein evidence and references

Localization	Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:1246084 to 1247982 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1899	1246084..1247982	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000002794

References cited on this page View Complete Literature Guide for MUS81

1)	Interthal H and Heyer WD (2000) MUS81 encodes a novel helix-hairpin-helix protein involved in the response to UV- and methylation-induced DNA damage in Saccharomyces cerevisiae. Mol Gen Genet 263(5):812-27
2)	Bastin-Shanower SA, et al. (2003) The mechanism of Mus81-Mms4 cleavage site selection distinguishes it from the homologous endonuclease Rad1-Rad10. Mol Cell Biol 23(10):3487-96
3)	Fu Y and Xiao W (2003) Functional domains required for the Saccharomyces cerevisiae Mus81-Mms4 endonuclease complex formation and nuclear localization. DNA Repair (Amst) 2(12):1435-47
4)	Oh SD, et al. (2008) RecQ helicase, Sgs1, and XPF family endonuclease, Mus81-Mms4, resolve aberrant joint molecules during meiotic recombination. Mol Cell 31(3):324-36
5)	Jessop L and Lichten M (2008) Mus81/Mms4 endonuclease and Sgs1 helicase collaborate to ensure proper recombination intermediate metabolism during meiosis. Mol Cell 31(3):313-23
6)	Gallo-Fernandez M, et al. (2012) Cell cycle-dependent regulation of the nuclease activity of Mus81-Eme1/Mms4. Nucleic Acids Res 40(17):8325-35