EFT2/YDR385W Summary Help

Standard Name EFT2 1
Systematic Name YDR385W
Feature Type ORF, Verified
Description Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description Elongation Factor Two 1
Chromosomal Location
ChrIV:1243230 to 1245758 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All EFT2 GO evidence and references
  View Computational GO annotations for EFT2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 15 genes
Resources
Classical genetics
conditional
overexpression
unspecified
Large-scale survey
null
overexpression
Resources
185 total interaction(s) for 131 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 71
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 2
  • PCA: 6
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 82
  • Positive Genetic: 8
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 842
Molecular Weight (Da) 93,288
Isoelectric Point (pI) 6.25
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1243230 to 1245758 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2529 1243230..1245758 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002793
References cited on this page View Complete Literature Guide for EFT2
1) Perentesis JP, et al.  (1992) Saccharomyces cerevisiae elongation factor 2. Genetic cloning, characterization of expression, and G-domain modeling. J Biol Chem 267(2):1190-7
2) Justice MC, et al.  (1998) Elongation factor 2 as a novel target for selective inhibition of fungal protein synthesis. J Biol Chem 273(6):3148-51
3) Dunlop PC and Bodley JW  (1983) Biosynthetic labeling of diphthamide in Saccharomyces cerevisiae. J Biol Chem 258(8):4754-8
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61