SEM1/YDR363W-A Summary Help

Standard Name SEM1 1
Systematic Name YDR363W-A
Alias DSS1 , HOD1
Feature Type ORF, Verified
Description Component of lid subcomplex of 26S proteasome regulatory subunit; involved in mRNA export mediated by TREX-2 complex (Sac3p-Thp1p); assumes different conformations in different contexts, functions as molecular glue stabilizing the Rpn3p/Rpn7p regulatory heterodimer, and tethers it to lid helical bundle; ortholog of human DSS1; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5, 6)
Name Description Suppressor of Exocyst Mutations 1
Chromosomal Location
ChrIV:1202128 to 1202397 | ORF Map | GBrowse
Gene Ontology Annotations All SEM1 GO evidence and references
  View Computational GO annotations for SEM1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Classical genetics
Large-scale survey
641 total interaction(s) for 345 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 98
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 32
  • Biochemical Activity: 1
  • Co-crystal Structure: 2
  • PCA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 5
  • Negative Genetic: 350
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 1
  • Positive Genetic: 86
  • Synthetic Growth Defect: 17
  • Synthetic Lethality: 32
  • Synthetic Rescue: 6

Expression Summary
Length (a.a.) 89
Molecular Weight (Da) 10,386
Isoelectric Point (pI) 4
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:1202128 to 1202397 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1999-07-17
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..270 1202128..1202397 2011-02-03 1999-07-17
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000007235
References cited on this page View Complete Literature Guide for SEM1
1) Jantti J, et al.  (1999) SEM1, a homologue of the split hand/split foot malformation candidate gene Dss1, regulates exocytosis and pseudohyphal differentiation in yeast. Proc Natl Acad Sci U S A 96(3):909-14
2) Sone T, et al.  (2004) Sem1p is a novel subunit of the 26 S proteasome from Saccharomyces cerevisiae. J Biol Chem 279(27):28807-16
3) Funakoshi M, et al.  (2004) Sem1, the yeast ortholog of a human BRCA2-binding protein, is a component of the proteasome regulatory particle that enhances proteasome stability. J Cell Sci 117(Pt 26):6447-54
4) Wilmes GM, et al.  (2008) A genetic interaction map of RNA-processing factors reveals links between Sem1/Dss1-containing complexes and mRNA export and splicing. Mol Cell 32(5):735-46
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6) Bohn S, et al.  (2013) Localization of the regulatory particle subunit Sem1 in the 26S proteasome. Biochem Biophys Res Commun 435(2):250-4