EAF1/YDR359C Summary Help

Standard Name EAF1 1
Systematic Name YDR359C
Alias VID21 2
Feature Type ORF, Verified
Description Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 (3, 4, 5)
Name Description Esa1p-Associated Factor 1
Chromosomal Location
ChrIV:1194884 to 1191936 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All EAF1 GO evidence and references
  View Computational GO annotations for EAF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
Large-scale survey
720 total interaction(s) for 462 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 67
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 35
  • Co-crystal Structure: 7
  • Co-localization: 1
  • Co-purification: 1
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Growth Defect: 31
  • Dosage Lethality: 27
  • Dosage Rescue: 1
  • Negative Genetic: 131
  • Phenotypic Enhancement: 1
  • Positive Genetic: 73
  • Synthetic Growth Defect: 237
  • Synthetic Lethality: 95
  • Synthetic Rescue: 5

Expression Summary
Length (a.a.) 982
Molecular Weight (Da) 112,501
Isoelectric Point (pI) 9.78
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:1194884 to 1191936 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 2004-02-06
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2949 1194884..1191936 2011-02-03 2004-02-06
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002767
References cited on this page View Complete Literature Guide for EAF1
1) Nourani A, et al.  (2001) Role of an ING1 growth regulator in transcriptional activation and targeted histone acetylation by the NuA4 complex. Mol Cell Biol 21(22):7629-40
2) Bonangelino CJ, et al.  (2002) Genomic screen for vacuolar protein sorting genes in Saccharomyces cerevisiae. Mol Biol Cell 13(7):2486-501
3) Nourani A, et al.  (2003) Opposite role of yeast ING family members in p53-dependent transcriptional activation. J Biol Chem 278(21):19171-5
4) Jordan PW, et al.  (2007) Novel roles for selected genes in meiotic DNA processing. PLoS Genet 3(12):e222
5) Auger A, et al.  (2008) Eaf1 is the platform for NuA4 molecular assembly that evolutionarily links chromatin acetylation to ATP-dependent exchange of histone H2A variants. Mol Cell Biol 28(7):2257-70