MSN5/YDR335W Summary Help

Standard Name MSN5
Systematic Name YDR335W
Alias KAP142 , STE21 1
Feature Type ORF, Verified
Description Karyopherin; involved in nuclear import and export of proteins, including import of replication protein A and export of Far1p and transcription factors Swi5p, Swi6p, Msn2p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm; exportin-5 homolog (2, 3, 4, 5, 6, 7, 8, 9, 10)
Name Description Multicopy suppressor of SNF1 mutation
Chromosomal Location
ChrIV:1141168 to 1144842 | ORF Map | GBrowse
Genetic position: 196 cM
Gene Ontology Annotations All MSN5 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
Large-scale survey
327 total interaction(s) for 225 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 23
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 17
  • Biochemical Activity: 1
  • Co-localization: 2
  • FRET: 9
  • Reconstituted Complex: 9
  • Two-hybrid: 16

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 3
  • Dosage Rescue: 4
  • Negative Genetic: 140
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 2
  • Positive Genetic: 37
  • Synthetic Growth Defect: 41
  • Synthetic Lethality: 11
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 1,224
Molecular Weight (Da) 142,116
Isoelectric Point (pI) 5.07
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:1141168 to 1144842 | ORF Map | GBrowse
Genetic position: 196 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3675 1141168..1144842 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002743
References cited on this page View Complete Literature Guide for MSN5
1) Akada R, et al.  (1996) Genetic relationships between the G protein beta gamma complex, Ste5p, Ste20p and Cdc42p: investigation of effector roles in the yeast pheromone response pathway. Genetics 143(1):103-17
2) Blondel M, et al.  (1999) Nuclear export of Far1p in response to pheromones requires the export receptor Msn5p/Ste21p. Genes Dev 13(17):2284-300
3) Kaffman A, et al.  (1998) The receptor Msn5 exports the phosphorylated transcription factor Pho4 out of the nucleus. Nature 396(6710):482-6
4) Alepuz PM, et al.  (1999) The Saccharomyces cerevisiae RanGTP-binding protein msn5p is involved in different signal transduction pathways. Genetics 153(3):1219-31
5) Yoshida K and Blobel G  (2001) The karyopherin Kap142p/Msn5p mediates nuclear import and nuclear export of different cargo proteins. J Cell Biol 152(4):729-40
6) Queralt E and Igual JC  (2003) Cell cycle activation of the Swi6p transcription factor is linked to nucleocytoplasmic shuttling. Mol Cell Biol 23(9):3126-40
7) Shibata S, et al.  (2006) Exportin-5 orthologues are functionally divergent among species. Nucleic Acids Res 34(17):4711-21
8) Murthi A, et al.  (2010) Regulation of tRNA Bidirectional Nuclear-Cytoplasmic Trafficking in Saccharomyces cerevisiae. Mol Biol Cell 21(4):639-49
9) Taberner FJ, et al.  (2012) Regulation of cell cycle transcription factor Swi5 by karyopherin Msn5. Biochim Biophys Acta 1823(4):959-70
10) Sunnaker M, et al.  (2013) Automatic generation of predictive dynamic models reveals nuclear phosphorylation as the key msn2 control mechanism. Sci Signal 6(277):ra41