MSN5/YDR335W Summary

MSN5/YDR335W Summary

Standard Name	MSN5
Systematic Name	YDR335W
Alias	KAP142 , STE21 1
Feature Type	ORF, Verified
Description	Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm (2, 3, 4, 5, 6, 7)
Name Description	Multicopy suppressor of SNF1 mutation

Chromosomal Location	ChrIV:1141168 to 1144842 \| ORF Map \| GBrowse

	Genetic position: 196 cM

Gene Ontology Annotations All MSN5 GO evidence and references


Molecular Function
Manually curated	importin-alpha export receptor activity (IDA, ISS) protein binding (IPI)
Biological Process
Manually curated	protein export from nucleus (IDA) tRNA re-export from nucleus (IGI)
Cellular Component
Manually curated	cytoplasm (IDA) nucleus (IDA)
High-throughput	integral to membrane (ISM)

Mutant phenotypes All MSN5 Phenotype evidence and references

Classical genetics
null	tRNA-His localization: abnormal nuclear export: absent Tef1p distribution: abnormal Yen1p distribution: abnormal
overexpression	nuclear morphology: abnormal
Large-scale survey
null	anaerobic growth: decreased auxotrophy cell size: increased chitin deposition: normal competitive fitness: decreased dessication resistance: decreased endocytosis: decreased Fus-Mid-GFP distribution: abnormal resistance to Calcofluor White: decreased resistance to quinine: decreased resistance to sirolimus: increased resistance to bleomycin: decreased resistance to methyl methanesulfonate: increased resistance to arsenite(3-): decreased resistance to calcium dichloride: decreased resistance to hygromycin B: decreased resistance to doxorubicin: decreased resistance to elesclomol-Cu: decreased resistance to cycloheximide: decreased resistance to camptothecin: decreased resistance to caffeine: decreased resistance to fluconazole: decreased resistance to wortmannin: normal respiratory growth: decreased rate stress resistance: decreased toxin resistance: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
overexpression	cell cycle progression: abnormal
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All MSN5 Interaction evidence and references

	319 total interaction(s) for 225 unique genes/features.
Physical Interactions	Affinity Capture-MS: 20 Affinity Capture-RNA: 1 Affinity Capture-Western: 16 Biochemical Activity: 1 Co-localization: 1 FRET: 9 Reconstituted Complex: 9 Two-hybrid: 16
Genetic Interactions	Dosage Growth Defect: 2 Dosage Lethality: 3 Dosage Rescue: 4 Negative Genetic: 139 Phenotypic Enhancement: 8 Phenotypic Suppression: 2 Positive Genetic: 37 Synthetic Growth Defect: 39 Synthetic Lethality: 11 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All MSN5 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:1141168 to 1144842 | |

SGD ORF map

: 196 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3675	1141168..1144842	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000002743

References cited on this page View Complete Literature Guide for MSN5

1)	Akada R, et al. (1996) Genetic relationships between the G protein beta gamma complex, Ste5p, Ste20p and Cdc42p: investigation of effector roles in the yeast pheromone response pathway. Genetics 143(1):103-17
2)	Blondel M, et al. (1999) Nuclear export of Far1p in response to pheromones requires the export receptor Msn5p/Ste21p. Genes Dev 13(17):2284-300
3)	Kaffman A, et al. (1998) The receptor Msn5 exports the phosphorylated transcription factor Pho4 out of the nucleus. Nature 396(6710):482-6
4)	Alepuz PM, et al. (1999) The Saccharomyces cerevisiae RanGTP-binding protein msn5p is involved in different signal transduction pathways. Genetics 153(3):1219-31
5)	Yoshida K and Blobel G (2001) The karyopherin Kap142p/Msn5p mediates nuclear import and nuclear export of different cargo proteins. J Cell Biol 152(4):729-40
6)	Queralt E and Igual JC (2003) Cell cycle activation of the Swi6p transcription factor is linked to nucleocytoplasmic shuttling. Mol Cell Biol 23(9):3126-40
7)	Murthi A, et al. (2010) Regulation of tRNA Bidirectional Nuclear-Cytoplasmic Trafficking in Saccharomyces cerevisiae. Mol Biol Cell 21(4):639-49