SAC3/YDR159W Summary Help

Standard Name SAC3 1
Systematic Name YDR159W
Alias LEP1
Feature Type ORF, Verified
Description mRNA export factor; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) (2, 3, 4, 5, 6, 7, 8, 9)
Name Description Suppressor of ACtin 2
Chromosomal Location
ChrIV:771877 to 775782 | ORF Map | GBrowse
Genetic position: 92 cM
Gene Ontology Annotations All SAC3 GO evidence and references
  View Computational GO annotations for SAC3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
Large-scale survey
726 total interaction(s) for 451 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 74
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 29
  • Biochemical Activity: 1
  • Co-crystal Structure: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 15

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 3
  • Negative Genetic: 408
  • Phenotypic Enhancement: 16
  • Phenotypic Suppression: 14
  • Positive Genetic: 92
  • Synthetic Growth Defect: 28
  • Synthetic Lethality: 27
  • Synthetic Rescue: 9

Expression Summary
Length (a.a.) 1,301
Molecular Weight (Da) 149,567
Isoelectric Point (pI) 9.54
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:771877 to 775782 | ORF Map | GBrowse
Genetic position: 92 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3906 771877..775782 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002566
References cited on this page View Complete Literature Guide for SAC3
1) Novick, P., et al.  (1989) Personal Communication, Mortimer Map Edition 10
2) Novick P, et al.  (1989) Suppressors of yeast actin mutations. Genetics 121(4):659-74
3) Fischer T, et al.  (2002) The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores. EMBO J 21(21):5843-52
4) Lei EP, et al.  (2003) Sac3 is an mRNA export factor that localizes to cytoplasmic fibrils of nuclear pore complex. Mol Biol Cell 14(3):836-47
5) Gallardo M, et al.  (2003) Nab2p and the Thp1p-Sac3p complex functionally interact at the interface between transcription and mRNA metabolism. J Biol Chem 278(26):24225-32
6) Li Z, et al.  (2009) Rational extension of the ribosome biogenesis pathway using network-guided genetics. PLoS Biol 7(10):e1000213
7) Chekanova JA, et al.  (2008) Sus1, Sac3, and Thp1 mediate post-transcriptional tethering of active genes to the nuclear rim as well as to non-nascent mRNP. RNA 14(1):66-77
8) Tous C, et al.  (2011) A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors. EMBO J 30(10):1953-64
9) Phimsen S, et al.  (2012) Selective cell death of p53-insufficient cancer cells is induced by knockdown of the mRNA export molecule GANP. Apoptosis 17(7):679-90