SWI5/YDR146C Summary Help

SWI5 BASIC INFORMATION

Standard Name SWI5 1
Systematic Name YDR146C
Feature Type ORF, Verified
Description Transcription factor that activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; localization to the nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase (2, 3, 4 and see Summary Paragraph)
Name Description SWItching deficient
GO Annotations All SWI5 GO evidence and references
    View Computational GO annotations for SWI5
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulatory Role
Binding motifs SWI5 Transcription Factor Binding Sites and References
  • KGCTGR
Mutant Phenotype All SWI5 Phenotype details and references
Classical genetics
null
unspecified
Large-scale survey
null
overexpression
Interactions SWI5 All interactions details and references
137 total interaction(s) for 111 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-Western: 6
  • Biochemical Activity: 4
  • Co-localization: 7
  • Reconstituted Complex: 10
  • Two-hybrid: 12
Genetic Interactions
  • Dosage Lethality: 3
  • Dosage Rescue: 5
  • Phenotypic Enhancement: 27
  • Phenotypic Suppression: 36
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 6
  • Synthetic Rescue: 11
Sequence Information
ChrIV:750740 to 748611 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2008-06-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2130 750740..748611 2008-06-05 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000002553

SWI5 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
 GBrowse
  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for SWI5

SUMMARY PARAGRAPH for SWI5

SWI5 encodes a transcription factor that activates transcription of genes expressed at the M/G1 boundary and in G1 phase of the cell cycle (3, 5, 6). The DNA binding domain of Swi5p is very similar to that of another G1-specific transcription activator, Ace2p (3). Swi5p and Ace2p bind the same DNA sequences in vitro with similar affinites, and regulate a shared set of genes in vivo (5, 6); however, the two proteins also show distinct promoter specificities in some instances (3, 6). For example, Swi5p activates transcription of the HO endonuclease gene, whereas Ace2p does not; conversely, CTS1 is activated by Ace2p and not by Swi5p (3). Distinct regions of Swi5p and Ace2p that are involved in promoter-specific transcription activation have been identified (6).

SWI5 is transcribed in G2 phase (7); cell cycle specific SWI5 expression requires Mcm1p and the uncloned "Swi Five Factor (SFF)" (8, 9). The localization of Swi5p is regulated by phosphorylation; dephosphorylated Swi5p is found in the nucleus, and therefore active, early in G1 phase (10, 2).

Last updated: 1999-12-03

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SWI5]

1) Stillman, D.  (1993) Personal Communication, Mortimer Map Edition 12
2) Moll T, et al.  (1991) The role of phosphorylation and the CDC28 protein kinase in cell cycle-regulated nuclear import of the S. cerevisiae transcription factor SWI5. Cell 66(4):743-58
3) Dohrmann PR, et al.  (1992) Parallel pathways of gene regulation: homologous regulators SWI5 and ACE2 differentially control transcription of HO and chitinase. Genes Dev 6(1):93-104
4) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
5) Dohrmann PR, et al.  (1996) Role of negative regulation in promoter specificity of the homologous transcriptional activators Ace2p and Swi5p. Mol Cell Biol 16(4):1746-58
6) McBride HJ, et al.  (1999) Distinct regions of the Swi5 and Ace2 transcription factors are required for specific gene activation. J Biol Chem 274(30):21029-36
7) Nasmyth K, et al.  (1987) Cell cycle regulation of SW15 is required for mother-cell-specific HO transcription in yeast. Cell 49(4):549-58
8) Lydall D, et al.  (1991) A new role for MCM1 in yeast: cell cycle regulation of SW15 transcription. Genes Dev 5(12B):2405-19
9) Althoefer H, et al.  (1995) Mcm1 is required to coordinate G2-specific transcription in Saccharomyces cerevisiae. Mol Cell Biol 15(11):5917-28
10) Nasmyth K, et al.  (1990) The identification of a second cell cycle control on the HO promoter in yeast: cell cycle regulation of SW15 nuclear entry. Cell 62(4):631-47