FOB1/YDR110W Summary Help

Standard Name FOB1 1
Systematic Name YDR110W
Alias HRM1 2
Feature Type ORF, Verified
Description Nucleolar protein that binds the rDNA replication fork barrier site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to replication fork barrier (RFB), and rDNA repeat segregation; related to retroviral integrases (1, 2, 3, 4, 5, 6)
Name Description FOrk Blocking less 1
Chromosomal Location
ChrIV:676102 to 677802 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All FOB1 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
null
overexpression
unspecified
Large-scale survey
null
overexpression
Resources
105 total interaction(s) for 65 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 19
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 9
  • Reconstituted Complex: 2
  • Two-hybrid: 17

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 11
  • Phenotypic Enhancement: 11
  • Phenotypic Suppression: 15
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 5
  • Synthetic Rescue: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 566
Molecular Weight (Da) 65,318
Isoelectric Point (pI) 6.12
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:676102 to 677802 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1701 676102..677802 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002517
References cited on this page View Complete Literature Guide for FOB1
1) Kobayashi T and Horiuchi T  (1996) A yeast gene product, Fob1 protein, required for both replication fork blocking and recombinational hotspot activities. Genes Cells 1(5):465-74
2) Defossez PA, et al.  (1999) Elimination of replication block protein Fob1 extends the life span of yeast mother cells. Mol Cell 3(4):447-55
3) Dlakic M  (2002) A model of the replication fork blocking protein Fob1p based on the catalytic core domain of retroviral integrases. Protein Sci 11(5):1274-7
4) Kobayashi T  (2003) The replication fork barrier site forms a unique structure with Fob1p and inhibits the replication fork. Mol Cell Biol 23(24):9178-88
5) Machin F, et al.  (2006) Transcription of ribosomal genes can cause nondisjunction. J Cell Biol 173(6):893-903
6) Johzuka K and Horiuchi T  (2009) The cis element and factors required for condensin recruitment to chromosomes. Mol Cell 34(1):26-35