GIS1/YDR096W Summary Help

Standard Name GIS1 1
Systematic Name YDR096W
Feature Type ORF, Verified
Description Histone demethylase and transcription factor; regulates genes during nutrient limitation; activity modulated by proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminus that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; relocalizes to the cytosol in response to hypoxia; GIS1 has a paralog, RPH1, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7, 8, 9)
Name Description GIg1-2 Suppressor 3
Chromosomal Location
ChrIV:637139 to 639823 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All GIS1 GO evidence and references
  View Computational GO annotations for GIS1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
102 total interaction(s) for 78 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Two-hybrid: 20

Genetic Interactions
  • Dosage Lethality: 3
  • Dosage Rescue: 8
  • Negative Genetic: 38
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 9
  • Positive Genetic: 2
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 1
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 894
Molecular Weight (Da) 99,480
Isoelectric Point (pI) 7.49
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:637139 to 639823 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2685 637139..639823 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002503
References cited on this page View Complete Literature Guide for GIS1
1) Jang YK, et al.  (1999) RPH1 and GIS1 are damage-responsive repressors of PHR1. Mol Cell Biol 19(11):7630-8
2) Pedruzzi I, et al.  (2000) Saccharomyces cerevisiae Ras/cAMP pathway controls post-diauxic shift element-dependent transcription through the zinc finger protein Gis1. EMBO J 19(11):2569-79
3) Balciunas D and Ronne H  (1999) Yeast genes GIS1-4: multicopy suppressors of the Gal- phenotype of snf1 mig1 srb8/10/11 cells. Mol Gen Genet 262(4-5):589-99
4) Oshiro J, et al.  (2003) Regulation of the yeast DPP1-encoded diacylglycerol pyrophosphate phosphatase by transcription factor Gis1p. J Biol Chem 278(34):31495-503
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Tu S, et al.  (2007) Identification of histone demethylases in Saccharomyces cerevisiae. J Biol Chem 282(19):14262-71
7) Zhang N and Oliver SG  (2010) The transcription activity of Gis1 is negatively modulated by proteasome-mediated limited proteolysis. J Biol Chem 285(9):6465-76
8) Quan Z, et al.  (2011) JmjN interacts with JmjC to ensure selective proteolysis of Gis1 by the proteasome. Microbiology 157(Pt 9):2694-701
9) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30
10) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87