RRP8/YDR083W Summary Help

Standard Name RRP8
Systematic Name YDR083W
Feature Type ORF, Verified
Description Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 (1, 2, 3, 4)
Name Description Ribosomal RNA Processing
Chromosomal Location
ChrIV:612073 to 613251 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RRP8 GO evidence and references
  View Computational GO annotations for RRP8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
repressible
Resources
140 total interaction(s) for 111 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 2

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 70
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 47
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 392
Molecular Weight (Da) 45,985
Isoelectric Point (pI) 10.21
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:612073 to 613251 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2006-01-19
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1179 612073..613251 2011-02-03 2006-01-19
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002490
References cited on this page View Complete Literature Guide for RRP8
1) Bousquet-Antonelli C, et al.  (2000) Rrp8p is a yeast nucleolar protein functionally linked to Gar1p and involved in pre-rRNA cleavage at site A2. RNA 6(6):826-43
2) Askree SH, et al.  (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
3) Ben-Shitrit T, et al.  (2012) Systematic identification of gene annotation errors in the widely used yeast mutation collections.LID - 10.1038/nmeth.1890 [doi] Nat Methods ()
4) Peifer C, et al.  (2013) Yeast Rrp8p, a novel methyltransferase responsible for m1A 645 base modification of 25S rRNA. Nucleic Acids Res 41(2):1151-63