NRG1/YDR043C Summary Help

Standard Name NRG1 1
Systematic Name YDR043C
Feature Type ORF, Verified
Description Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; NRG1 has a paralog, NRG2, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description Negative Regulator of Glucose-repressed genes 1, 2
Chromosomal Location
ChrIV:543369 to 542674 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NRG1 GO evidence and references
  View Computational GO annotations for NRG1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 94 genes
Regulators 9 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
177 total interaction(s) for 142 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 5
  • Biochemical Activity: 2
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 8
  • Dosage Rescue: 1
  • Negative Genetic: 104
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 2
  • Positive Genetic: 35
  • Synthetic Growth Defect: 2
  • Synthetic Rescue: 11

Resources
Expression Summary
histogram
Resources
Length (a.a.) 231
Molecular Weight (Da) 26,743
Isoelectric Point (pI) 9.01
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:543369 to 542674 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..696 543369..542674 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002450
References cited on this page View Complete Literature Guide for NRG1
1) Zhou H and Winston F  (2001) NRG1 is required for glucose repression of the SUC2 and GAL genes of Saccharomyces cerevisiae. BMC Genet 2():5
2) Park SH, et al.  (1999) Nrg1 is a transcriptional repressor for glucose repression of STA1 gene expression in Saccharomyces cerevisiae. Mol Cell Biol 19(3):2044-50
3) Kuchin S, et al.  (2002) Snf1 protein kinase and the repressors Nrg1 and Nrg2 regulate FLO11, haploid invasive growth, and diploid pseudohyphal differentiation. Mol Cell Biol 22(12):3994-4000
4) Lamb TM and Mitchell AP  (2003) The transcription factor Rim101p governs ion tolerance and cell differentiation by direct repression of the regulatory genes NRG1 and SMP1 in Saccharomyces cerevisiae. Mol Cell Biol 23(2):677-86
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104
7) Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66