LRG1/YDL240W Summary Help

Standard Name LRG1 1
Systematic Name YDL240W
Feature Type ORF, Verified
Description GTPase-activating protein (GAP); contains Rho1p-specific GAP activity, interacting with activated forms of Rho1p; functions along with Sac7p as a negative regulator of the Pkc1p-mediated cell wall integrity signaling pathway; negative regulator of cell wall 1,3-beta-glucan biosynthesis; required for efficient cell fusion; contains a RhoGAP domain and three Lin-11-Isl1-Mec-3 (LIM) domains (2, 3, 4)
Name Description Lim-RhoGap homolog 1
Chromosomal Location
ChrIV:22823 to 25876 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All LRG1 GO evidence and references
  View Computational GO annotations for LRG1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
overexpression
unspecified
Large-scale survey
null
overexpression
Resources
67 total interaction(s) for 53 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Biochemical Activity: 1
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 2
  • Dosage Rescue: 5
  • Negative Genetic: 23
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 1
  • Positive Genetic: 5
  • Synthetic Lethality: 1
  • Synthetic Rescue: 15

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,017
Molecular Weight (Da) 116,651
Isoelectric Point (pI) 8.32
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:22823 to 25876 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3054 22823..25876 1996-07-31 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002399
References cited on this page View Complete Literature Guide for LRG1
1) Muller L, et al.  (1994) LRG1 is expressed during sporulation in Saccharomyces cerevisiae and contains motifs similar to LIM and rho/racGAP domains. Nucleic Acids Res 22(15):3151-4
2) Watanabe D, et al.  (2001) Yeast Lrg1p acts as a specialized RhoGAP regulating 1,3-beta-glucan synthesis. Yeast 18(10):943-51
3) Lorberg A, et al.  (2001) Lrg1p functions as a putative GTPase-activating protein in the Pkc1p-mediated cell integrity pathway in Saccharomyces cerevisiae. Mol Genet Genomics 266(3):514-26
4) Fitch PG, et al.  (2004) Lrg1p Is a Rho1 GTPase-activating protein required for efficient cell fusion in yeast. Genetics 168(2):733-46