GLE1/YDL207W Summary Help

Standard Name GLE1 1, 2
Systematic Name YDL207W
Alias RSS1 3 , BRR3 4 , NLE2 5
Feature Type ORF, Verified
Description Cytoplasmic nucleoporin required for polyadenylated mRNA export; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export; mediates translation initiation; required for efficient translation termination (1, 3, 6, 7, 8, 9, 10)
Name Description GLFG (glycine-leucine-phenylalanine-glycine) LEthal 2
Chromosomal Location
ChrIV:88248 to 89864 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All GLE1 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
conditional
Large-scale survey
null
overexpression
reduction of function
Resources
110 total interaction(s) for 52 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 28
  • Affinity Capture-Western: 6
  • Biochemical Activity: 2
  • Co-crystal Structure: 1
  • Co-purification: 1
  • FRET: 1
  • PCA: 2
  • Reconstituted Complex: 13
  • Two-hybrid: 14

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Dosage Rescue: 11
  • Negative Genetic: 1
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 13
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 538
Molecular Weight (Da) 62,073
Isoelectric Point (pI) 9.58
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:88248 to 89864 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1617 88248..89864 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002366
References cited on this page View Complete Literature Guide for GLE1
1) Murphy R and Wente SR  (1996) An RNA-export mediator with an essential nuclear export signal. Nature 383(6598):357-60
2) Murphy R, et al.  (1996) GLE2, a Saccharomyces cerevisiae homologue of the Schizosaccharomyces pombe export factor RAE1, is required for nuclear pore complex structure and function. Mol Biol Cell 7(12):1921-37
3) Del Priore V, et al.  (1996) The product of the Saccharomyces cerevisiae RSS1 gene, identified as a high-copy suppressor of the rat7-1 temperature-sensitive allele of the RAT7/NUP159 nucleoporin, is required for efficient mRNA export. Mol Biol Cell 7(10):1601-21
4) Noble SM and Guthrie C  (1996) Identification of novel genes required for yeast pre-mRNA splicing by means of cold-sensitive mutations. Genetics 143(1):67-80
5) Harper NC, et al.  (2008) Mutations affecting spindle pole body and mitotic exit network function are synthetically lethal with a deletion of the nucleoporin NUP1 in S. cerevisiae. Curr Genet 53(2):95-105
6) Hurwitz ME, et al.  (1998) Two yeast nuclear pore complex proteins involved in mRNA export form a cytoplasmically oriented subcomplex. Proc Natl Acad Sci U S A 95(19):11241-5
7) Alcazar-Roman AR, et al.  (2006) Inositol hexakisphosphate and Gle1 activate the DEAD-box protein Dbp5 for nuclear mRNA export. Nat Cell Biol 8(7):711-6
8) Weirich CS, et al.  (2006) Activation of the DExD/H-box protein Dbp5 by the nuclear-pore protein Gle1 and its coactivator InsP6 is required for mRNA export. Nat Cell Biol 8(7):668-76
9) Bolger TA, et al.  (2008) The mRNA export factor Gle1 and inositol hexakisphosphate regulate distinct stages of translation. Cell 134(4):624-33
10) Noble KN, et al.  (2011) The Dbp5 cycle at the nuclear pore complex during mRNA export II: nucleotide cycling and mRNP remodeling by Dbp5 are controlled by Nup159 and Gle1. Genes Dev 25(10):1065-77