DHH1/YDL160C Summary Help

Standard Name DHH1 1
Systematic Name YDL160C
Feature Type ORF, Verified
Description Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, role in translational repression, mRNA decay, and processing body dynamics; may have a role in mRNA export; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress (2, 3, 4, 5, 6, 7 and see Summary Paragraph)
Name Description DEAD box Helicase Homolog 1
Chromosomal Location
ChrIV:171930 to 170410 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All DHH1 GO evidence and references
  View Computational GO annotations for DHH1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 9 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
502 total interaction(s) for 394 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 68
  • Affinity Capture-RNA: 298
  • Affinity Capture-Western: 13
  • Co-purification: 1
  • PCA: 25
  • Protein-RNA: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 14

Genetic Interactions
  • Dosage Growth Defect: 4
  • Dosage Lethality: 8
  • Dosage Rescue: 13
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 11
  • Synthetic Growth Defect: 20
  • Synthetic Lethality: 9
  • Synthetic Rescue: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 506
Molecular Weight (Da) 57,544
Isoelectric Point (pI) 7.97
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:171930 to 170410 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1521 171930..170410 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002319
SUMMARY PARAGRAPH for DHH1

DHH1 encodes a highly conserved DEAD-box RNA helicase that associates with components of mRNA decapping, deadenylation, and transcription complexes (2, 8, 9). Dhh1p stimulates mRNA decapping by decapping enzyme Dcp1p, and has been shown to localize to discrete cytoplasmic foci known as P-bodies, which appear to be involved in sequestering mRNAs in a nontranslating pool, from which they are subsequently degraded or possibly reactivated for translation (10). Orthologs of Dhh1p in other eukaryotes, such as Xenopus and Drosophila, play roles in repressing translation of specific mRNAs during early development (11, 12).

DHH1 in yeast is important in release from DNA-damage-induced cell cycle arrest at the G1/S checkpoint (4). dhh1 mutants are not defective for DNA repair and recover normally from the G2/M and replication checkpoints, suggesting a specific function for Dhh1p in recovery from G1/S checkpoint arrest (4). Dhh1p has been suggested to play a role in partitioning mRNAs between translatable and nontranslatable pools, which has been implicated in the recovery from G1/S cell cycle arrest following DNA damage (4). DHH1 is orthologous to the human putative proto-oncogene p54/RCK, and the high degree of conservation between these orthologs suggests that this mechanism is conserved among all eukaryotes and potentially important in human disease (4).

Last updated: 2005-08-06 Contact SGD

References cited on this page View Complete Literature Guide for DHH1
1) Strahl-Bolsinger S and Tanner W  (1993) A yeast gene encoding a putative RNA helicase of the "DEAD"-box family. Yeast 9(4):429-32
2) Coller JM, et al.  (2001) The DEAD box helicase, Dhh1p, functions in mRNA decapping and interacts with both the decapping and deadenylase complexes. RNA 7(12):1717-27
3) Tseng-Rogenski SS, et al.  (2003) Functional conservation of Dhh1p, a cytoplasmic DExD/H-box protein present in large complexes. Nucleic Acids Res 31(17):4995-5002
4) Bergkessel M and Reese JC  (2004) An essential role for the Saccharomyces cerevisiae DEAD-box helicase DHH1 in G1/S DNA-damage checkpoint recovery. Genetics 167(1):21-33
5) Carroll JS, et al.  (2011) The DExD/H box ATPase Dhh1 functions in translational repression, mRNA decay, and processing body dynamics. J Cell Biol 194(4):527-37
6) Chang LC and Lee FJ  (2012) The RNA helicase Dhh1p cooperates with Rbp1p to promote porin mRNA decay via its non-conserved C-terminal domain. Nucleic Acids Res 40(3):1331-44
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
8) Fischer N and Weis K  (2002) The DEAD box protein Dhh1 stimulates the decapping enzyme Dcp1. EMBO J 21(11):2788-97
9) Maillet L and Collart MA  (2002) Interaction between Not1p, a component of the Ccr4-not complex, a global regulator of transcription, and Dhh1p, a putative RNA helicase. J Biol Chem 277(4):2835-42
10) Sheth U and Parker R  (2003) Decapping and decay of messenger RNA occur in cytoplasmic processing bodies. Science 300(5620):805-8
11) Smillie DA and Sommerville J  (2002) RNA helicase p54 (DDX6) is a shuttling protein involved in nuclear assembly of stored mRNP particles. J Cell Sci 115(Pt 2):395-407
12) Nakamura A, et al.  (2001) Me31B silences translation of oocyte-localizing RNAs through the formation of cytoplasmic RNP complex during Drosophila oogenesis. Development 128(17):3233-42