SCM3/YDL139C Summary Help

Standard Name SCM3 1
Systematic Name YDL139C
Feature Type ORF, Verified
Description Nonhistone component of centromeric chromatin; binds to histone H3 variant, Cse4p, and recruits it to centromeres; involved in the assembly and maintenance of Cse4-H4 at centromeres; required for kinetochore assembly and G2/M progression; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP (1, 2, 3, 4, 5, 6, 7)
Name Description Suppressor of Chromosome Missegregation 2
Chromosomal Location
ChrIV:212046 to 211375 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SCM3 GO evidence and references
  View Computational GO annotations for SCM3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
conditional
null
repressible
Large-scale survey
null
overexpression
reduction of function
Resources
53 total interaction(s) for 29 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 9
  • Biochemical Activity: 1
  • Co-localization: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 15

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 7
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 3
  • Synthetic Growth Defect: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 223
Molecular Weight (Da) 25,754
Isoelectric Point (pI) 6.65
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:212046 to 211375 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2003-09-22
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..672 212046..211375 2011-02-03 2003-09-22
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002298
References cited on this page View Complete Literature Guide for SCM3
1) Chen Y, et al.  (2000) The N terminus of the centromere H3-like protein Cse4p performs an essential function distinct from that of the histone fold domain. Mol Cell Biol 20(18):7037-48
2) Stoler S, et al.  (2007) Scm3, an essential Saccharomyces cerevisiae centromere protein required for G2/M progression and Cse4 localization. Proc Natl Acad Sci U S A 104(25):10571-6
3) Camahort R, et al.  (2007) Scm3 is essential to recruit the histone h3 variant cse4 to centromeres and to maintain a functional kinetochore. Mol Cell 26(6):853-65
4) Mizuguchi G, et al.  (2007) Nonhistone Scm3 and histones CenH3-H4 assemble the core of centromere-specific nucleosomes. Cell 129(6):1153-64
5) Aravind L, et al.  (2007) Domain architectures of the Scm3p protein provide insights into centromere function and evolution. Cell Cycle 6(20):2511-5
6) Hewawasam G, et al.  (2010) Psh1 Is an E3 Ubiquitin Ligase that Targets the Centromeric Histone Variant Cse4. Mol Cell 40(3):444-54
7) Mishra PK, et al.  (2011) Misregulation of Scm3p/HJURP Causes Chromosome Instability in Saccharomyces cerevisiae and Human Cells. PLoS Genet 7(9):e1002303