IWR1/YDL115C Summary

IWR1/YDL115C Summary

Standard Name	IWR1 1
Systematic Name	YDL115C
Feature Type	ORF, Verified
Description	RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress (1, 2, 3, 4, 5, 6)
Name Description	Interacts With RNA polymerase II 2

Chromosomal Location	ChrIV:255126 to 253995 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All IWR1 GO evidence and references

	View Computational GO annotations for IWR1
Molecular Function
Manually curated	protein binding (IPI)
Biological Process
Manually curated	nucleocytoplasmic transport (IMP) RNA polymerase II complex localization to nucleus (IMP) RNA polymerase III complex localization to nucleus (IMP)
Cellular Component
Manually curated	cytoplasm (IDA) DNA-directed RNA polymerase II, holoenzyme (IPI) nucleus (IDA)
High-throughput	cytoplasm (IDA) nucleus (IDA)

Mutant phenotypes All IWR1 Phenotype evidence and references

Classical genetics
null	ARG1 mRNA accumulation: increased GAL10 mRNA accumulation: decreased killer toxin resistance: decreased resistance to calcium dichloride: decreased resistance to Echinocandin B: decreased
Large-scale survey
null	bud morphology: abnormal competitive fitness: decreased dessication resistance: decreased fermentative growth: decreased rate heat sensitivity: increased oxidative stress resistance: decreased resistance to L-1,4-dithiothreitol: decreased resistance to ethanol: decreased resistance to doxorubicin: decreased resistance to acrolein: decreased sporulation: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All IWR1 Interaction evidence and references

	252 total interaction(s) for 192 unique genes/features.
Physical Interactions	Affinity Capture-MS: 51 Affinity Capture-RNA: 2 Affinity Capture-Western: 2 Biochemical Activity: 4 Co-localization: 2 Reconstituted Complex: 1
Genetic Interactions	Negative Genetic: 126 Phenotypic Enhancement: 2 Phenotypic Suppression: 13 Positive Genetic: 24 Synthetic Growth Defect: 6 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 11 Synthetic Rescue: 7
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All IWR1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:255126 to 253995 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2007-07-10 | Sequence: 2007-07-10

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..82	255126..255045	2007-07-10	2007-07-10
Intron	83..152	255044..254975	2007-07-10	2007-07-10
CDS	153..1132	254974..253995	2007-07-10	2007-07-10

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000002273

References cited on this page View Complete Literature Guide for IWR1

1)	Page N, et al. (2003) A Saccharomyces cerevisiae genome-wide mutant screen for altered sensitivity to K1 killer toxin. Genetics 163(3):875-94
2)	Krogan NJ, et al. (2006) Global landscape of protein complexes in the yeast Saccharomyces cerevisiae. Nature 440(7084):637-43
3)	Peiro-Chova L and Estruch F (2009) The yeast RNA polymerase II-associated factor Iwr1p is involved in the basal and regulated transcription of specific genes. J Biol Chem 284(42):28958-67
4)	Czeko E, et al. (2011) Iwr1 Directs RNA Polymerase II Nuclear Import. Mol Cell 42(2):261-6
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6)	Minaker SW, et al. (2013) Biogenesis of RNA Polymerases II and III Requires the Conserved GPN Small GTPases in Saccharomyces cerevisiae. Genetics 193(3):853-64