Standard Name	KIN28 1
Systematic Name	YDL108W
Feature Type	ORF, Verified
Description	Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters (2, 3)
Name Description	protein KINase

Chromosomal Location	ChrIV:267698 to 268699 | ORF Map | GBrowse

	View Computational GO annotations for KIN28
Molecular Function
Manually curated	contributes_to core RNA polymerase binding transcription factor activity (IC) NOT cyclin-dependent protein kinase activating kinase activity (IDA, IMP) RNA polymerase II carboxy-terminal domain kinase activity (IDA, IMP)
High-throughput	protein kinase activity (IDA)
Biological Process
Manually curated	7-methylguanosine mRNA capping (IMP) phosphorylation of RNA polymerase II C-terminal domain (IDA, IMP) phosphorylation of RNA polymerase II C-terminal domain involved in recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex (IMP, IPI) phosphorylation of RNA polymerase II C-terminal domain serine 5 residues involved in recruitment of mRNA capping enzyme to RNA polymerase II holoenzyme complex (IMP) positive regulation of transcription from RNA polymerase II promoter (IDA) transcription from RNA polymerase I promoter (IMP) transcription from RNA polymerase II promoter (IDA, IMP)
High-throughput	protein phosphorylation (IDA)
Cellular Component
Manually curated	holo TFIIH complex (IDA) TFIIK complex (IDA)

Classical genetics
conditional	resistance to cordycepin: decreased
null	inviable
Large-scale survey
null	inviable
reduction of function	competitive fitness: decreased
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	129 total interaction(s) for 79 unique genes/features.
Physical Interactions	Affinity Capture-MS: 14 Affinity Capture-Western: 11 Biochemical Activity: 19 Co-crystal Structure: 1 Co-localization: 2 Co-purification: 8 Far Western: 2 Reconstituted Complex: 21 Two-hybrid: 7
Genetic Interactions	Dosage Lethality: 2 Dosage Rescue: 8 Negative Genetic: 6 Positive Genetic: 7 Synthetic Growth Defect: 6 Synthetic Lethality: 13 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIV:267698 to 268699 | ORF Map | GBrowse
Last Update	Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..28 267698..267725 2004-02-11 1996-07-31 Intron 29..109 267726..267806 2004-02-11 1996-07-31 CDS 110..1002 267807..268699 2004-02-11 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS) Intron (29 - 109)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000002266