SUB2/YDL084W Summary Help

Standard Name SUB2
Systematic Name YDL084W
Feature Type ORF, Verified
Description Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress (1, 2, 3, 4, 5)
Name Description SUppressor of Brr1-1
Chromosomal Location
ChrIV:305237 to 306577 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SUB2 GO evidence and references
  View Computational GO annotations for SUB2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 6 genes
Resources
Classical genetics
null
reduction of function
repressible
Large-scale survey
conditional
null
overexpression
Resources
189 total interaction(s) for 95 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 65
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 21
  • Co-localization: 1
  • Co-purification: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 9

Genetic Interactions
  • Dosage Growth Defect: 17
  • Dosage Lethality: 2
  • Dosage Rescue: 13
  • Negative Genetic: 23
  • Phenotypic Enhancement: 1
  • Positive Genetic: 2
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 17
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 446
Molecular Weight (Da) 50,309
Isoelectric Point (pI) 5.29
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:305237 to 306577 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1341 305237..306577 2004-02-11 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002242
References cited on this page View Complete Literature Guide for SUB2
1) Fan HY, et al.  (2001) High-copy-number expression of Sub2p, a member of the RNA helicase superfamily, suppresses hpr1-mediated genomic instability. Mol Cell Biol 21(16):5459-70
2) Jensen TH, et al.  (2001) The DECD box putative ATPase Sub2p is an early mRNA export factor. Curr Biol 11(21):1711-5
3) Strasser K and Hurt E  (2001) Splicing factor Sub2p is required for nuclear mRNA export through its interaction with Yra1p. Nature 413(6856):648-52
4) Strasser K, et al.  (2002) TREX is a conserved complex coupling transcription with messenger RNA export. Nature 417(6886):304-8
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76