Standard Name	SLM3 1
Systematic Name	YDL033C
Alias	MTO2 2 , MTU1 3
Feature Type	ORF, Verified
Description	tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) (2, 3)
Name Description	Synthetic Lethal with Mss4 1

Chromosomal Location	ChrIV:393912 to 392659 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for SLM3
Molecular Function
Manually curated	tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase activity (IMP, ISS)
Biological Process
Manually curated	mitochondrial tRNA thio-modification (IMP)
Cellular Component
Manually curated	mitochondrion (IDA)
High-throughput	mitochondrion (IDA)

Classical genetics
null	mt tRNA accumulation: decreased mt tRNA-Lys modification: absent mt tRNA modification: decreased tellurium atom accumulation: increased nutrient utilization: decreased petite respiratory growth: decreased
Large-scale survey
null	auxotrophy chitin deposition: normal competitive fitness: decreased heat sensitivity: increased resistance to amitrole: decreased resistance to cycloheximide: decreased resistance to sirolimus: decreased resistance to caffeine: increased resistance to fenpropimorph: increased resistance to fluconazole: increased resistance to tunicamycin: increased resistance to Calcofluor White: normal resistance to bleomycin: increased respiratory growth: absent respiratory growth: decreased rate sporulation: decreased starvation resistance: decreased vegetative growth: decreased rate viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	161 total interaction(s) for 150 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-RNA: 1 Biochemical Activity: 1
Genetic Interactions	Negative Genetic: 108 Phenotypic Enhancement: 2 Positive Genetic: 28 Synthetic Growth Defect: 9 Synthetic Lethality: 9 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIV:393912 to 392659 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1254 393912..392659 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000002191