MPS1/YDL028C Summary Help

Standard Name MPS1 1
Systematic Name YDL028C
Alias RPK1 , PAC8 2
Feature Type ORF, Verified
Description Dual-specificity kinase; autophosphorylation required for function; required for spindle pole body (SPB) duplication and spindle checkpoint function; contributes to bi-orientation by promoting formation of force-generating kinetochore-microtubule attachments in meiosis I; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p; substrate of APCC(Cdh1); similar to human Mps1p (3, 4, 5, 6, 7, 8, 9, 10, 11 and see Summary Paragraph)
Name Description MonoPolar Spindle 1
Chromosomal Location
ChrIV:403291 to 400997 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All MPS1 GO evidence and references
  View Computational GO annotations for MPS1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
Classical genetics
conditional
null
overexpression
repressible
unspecified
Large-scale survey
conditional
null
overexpression
reduction of function
Resources
210 total interaction(s) for 160 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 37
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 9
  • Biochemical Activity: 22
  • Reconstituted Complex: 18
  • Two-hybrid: 10

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Lethality: 12
  • Dosage Rescue: 8
  • Negative Genetic: 52
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 5
  • Positive Genetic: 12
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 13
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 764
Molecular Weight (Da) 86,771
Isoelectric Point (pI) 9.36
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:403291 to 400997 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2295 403291..400997 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002186
SUMMARY PARAGRAPH for MPS1

MPS1 encodes a dual-specificity protein kinase that is involved in spindle pole body (SPB) duplication and the spindle checkpoint (1, 3, 12). The SPB is the microtubule organizing center in yeast, and is the functional equivalent of the mammalian centrosome (13). Mps1p binds and phosphorylates Mob1p, which is required for completion of mitosis and may be required for the initiation of SPB duplication (4). The integral spindle pole body component Spc42p is phosphorylated by Mps1p, and this phosphorylation is required for proper assembly of Spc42p into the SPB (7). Spc98p, a component of the SPB, appears to be another substrate of Mps1p kinase activity (14).

The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules (see 15 for review; reference 16 describes defects detected by the checkpoint). Mps1p phosphorylates the checkpoint protein Mad1p, and overexpression of MPS1 arrests cells in mitosis (5). Mps1p, Bub1p, Bub3p, Mad1p, Mad2p, and Mad3p act in a branch of the spindle checkpoint pathway that may prevent premature chromosome disjunction. A second branch involves Bub2p and Bfa1p, and may prevent cytokinesis prior to chromosome segregation (17, 18, 19, 20). The Mps1p kinase domain shows sequence similarity to dual-specificity protein kinases from other species including mammalian esk and PYT/TTK (21, 22). An Mps1p-related kinase, Mph1, is involved in the spindle checkpoint in S. pombe (23).

Last updated: 2000-04-05 Contact SGD

References cited on this page View Complete Literature Guide for MPS1
1) Winey M, et al.  (1991) MPS1 and MPS2: novel yeast genes defining distinct steps of spindle pole body duplication. J Cell Biol 114(4):745-54
2) Geiser JR, et al.  (1997) Saccharomyces cerevisiae genes required in the absence of the CIN8-encoded spindle motor act in functionally diverse mitotic pathways. Mol Biol Cell 8(6):1035-50
3) Lauze E, et al.  (1995) Yeast spindle pole body duplication gene MPS1 encodes an essential dual specificity protein kinase. EMBO J 14(8):1655-63
4) Luca FC and Winey M  (1998) MOB1, an essential yeast gene required for completion of mitosis and maintenance of ploidy. Mol Biol Cell 9(1):29-46
5) Hardwick KG, et al.  (1996) Activation of the budding yeast spindle assembly checkpoint without mitotic spindle disruption. Science 273(5277):953-6
6) Friedman DB, et al.  (2001) Yeast Mps1p phosphorylates the spindle pole component Spc110p in the N-terminal domain. J Biol Chem 276(21):17958-67
7) Castillo AR, et al.  (2002) The yeast protein kinase Mps1p is required for assembly of the integral spindle pole body component Spc42p. J Cell Biol 156(3):453-65
8) Mattison CP, et al.  (2007) Mps1 activation loop autophosphorylation enhances kinase activity. J Biol Chem 282(42):30553-61
9) Ostapenko D, et al.  (2012) Identification of anaphase promoting complex substrates in S. cerevisiae. PLoS One 7(9):e45895
10) Meyer RE, et al.  (2013) Mps1 and Ipl1/Aurora B act sequentially to correctly orient chromosomes on the meiotic spindle of budding yeast. Science 339(6123):1071-4
11) Malvezzi F, et al.  (2013) A structural basis for kinetochore recruitment of the Ndc80 complex via two distinct centromere receptors. EMBO J 32(3):409-23
12) Weiss E and Winey M  (1996) The Saccharomyces cerevisiae spindle pole body duplication gene MPS1 is part of a mitotic checkpoint. J Cell Biol 132(1-2):111-23
13) Botstein D, et al.  (1997) "The yeast cytoskeleton." Pp. 1-90 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
14) Pereira G, et al.  (1998) Spc98p directs the yeast gamma-tubulin complex into the nucleus and is subject to cell cycle-dependent phosphorylation on the nuclear side of the spindle pole body. Mol Biol Cell 9(4):775-93
15) Hardwick KG  (1998) The spindle checkpoint. Trends Genet 14(1):1-4
16) Hardwick KG, et al.  (1999) Lesions in many different spindle components activate the spindle checkpoint in the budding yeast Saccharomyces cerevisiae. Genetics 152(2):509-18
17) Alexandru G, et al.  (1999) Sister chromatid separation and chromosome re-duplication are regulated by different mechanisms in response to spindle damage. EMBO J 18(10):2707-21
18) Fesquet D, et al.  (1999) A Bub2p-dependent spindle checkpoint pathway regulates the Dbf2p kinase in budding yeast. EMBO J 18(9):2424-34
19) Fraschini R, et al.  (1999) Budding yeast Bub2 is localized at spindle pole bodies and activates the mitotic checkpoint via a different pathway from Mad2. J Cell Biol 145(5):979-91
20) Li R  (1999) Bifurcation of the mitotic checkpoint pathway in budding yeast. Proc Natl Acad Sci U S A 96(9):4989-94
21) Poch O, et al.  (1994) RPK1, an essential yeast protein kinase involved in the regulation of the onset of mitosis, shows homology to mammalian dual-specificity kinases. Mol Gen Genet 243(6):641-53
22) Schutz AR and Winey M  (1998) New alleles of the yeast MPS1 gene reveal multiple requirements in spindle pole body duplication. Mol Biol Cell 9(4):759-74
23) He X, et al.  (1998) Mph1, a member of the Mps1-like family of dual specificity protein kinases, is required for the spindle checkpoint in S. pombe. J Cell Sci 111 ( Pt 12)():1635-47